Strong Taylor C, Thomas Jeffrey H
Genesis. 2011 Dec;49(12):912-8. doi: 10.1002/dvg.20783. Epub 2011 Aug 5.
Cellularization of the multinucleate Drosophila embryo occurs shortly after zygotic transcription begins. During cellular blastoderm morphogenesis, the microfilament cytoskeleton undergoes extensive reorganization. This cytoskeletal reorganization includes synchronized microfilament contraction regulated by src64, a gene encoding a Src nonreceptor tyrosine kinase. We report that src64 is maternally expressed in the Drosophila embryo and acts primarily as a maternal gene during cellularization. However, we show that src64 has some zygotic activity during late cellularization. By using compound chromosomes to generate embryos with wild-type levels of maternal src64 activity, we show that this zygotic activity is normally nonessential. We also report the identification of an alternate src64 transcript. Expression of this transcript is not affected by the src64Δ17 deletion mutation, explaining the presence of low levels of src64 activity observed in src64Δ17 mutants.
多核果蝇胚胎的细胞化在合子转录开始后不久就会发生。在细胞胚盘形态发生过程中，微丝细胞骨架会经历广泛的重组。这种细胞骨架重组包括由src64调控的同步微丝收缩，src64是一个编码Src非受体酪氨酸激酶的基因。我们报告称，src64在果蝇胚胎中由母体表达，并且在细胞化过程中主要作为一个母体基因发挥作用。然而，我们表明src64在细胞化后期具有一些合子活性。通过使用复合染色体来产生具有野生型水平母体src64活性的胚胎，我们表明这种合子活性通常是不必要的。我们还报告了对一种替代性src64转录本的鉴定。该转录本的表达不受src64Δ17缺失突变的影响，这解释了在src64Δ17突变体中观察到的低水平src64活性的存在。
