显微镜检查与半巢式多重聚合酶链反应诊断间日疟原虫和恶性疟原虫的比较。
Comparison of microscopical examination and semi-nested multiplex polymerase chain reaction in diagnosis of Plasmodium falciparum and P. vivax.
机构信息
Department of Medical Parasitology and Mycology, School of Public Health, Tehron University of Medical Sciences, Tehran, Islamic Republic of Iran.
出版信息
East Mediterr Health J. 2011 Jan;17(1):51-5.
We compared light microscopy examination and a semi-nested multiplex PCR (SnM-PCR) assay in endemic areas of the Islamic Republic of Iran. A total of 68 individuals with malaria-positive and suspected malaria symptoms were included in the study. Giemsa-stained thick blood films were examined under a light microscope for malaria parasites in 100 and 200 fields. DNA was extracted from blood samples and SnM-PCR based on the amplification of the small sub-unit ribosomal RNA (ssrRNA) gene sequences was applied. Microscopical examination showed that 48.5% (33.8% P. vivax and 14.7% P.falciparum) and 50% (35.3% P. vivax and 14.7% P. falciparum) of the samples were positive in 100 and 200 fields respectively. SnM-PCR showed the same results as the 200 field microscopy.
我们在伊朗伊斯兰共和国的流行地区比较了光学显微镜检查和半巢式多重 PCR（SnM-PCR）检测。共有 68 名疟疾病例阳性和疑似疟疾症状的个体纳入研究。在 100 和 200 个视野下用 Giemsa 染色的厚血涂片检查疟原虫。从血液样本中提取 DNA，并应用基于小亚基核糖体 RNA（ssrRNA）基因序列扩增的 SnM-PCR。显微镜检查显示，在 100 和 200 个视野下，分别有 48.5%（33.8%间日疟原虫和 14.7%恶性疟原虫）和 50%（35.3%间日疟原虫和 14.7%恶性疟原虫）的样本为阳性。SnM-PCR 与 200 视野显微镜检查结果相同。
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