Ebrahimzadeh A, Fouladi B, Fazaeli A
Department of Parasitology and Mycology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Parasitol Int. 2007 Mar;56(1):61-4. doi: 10.1016/j.parint.2006.12.001. Epub 2007 Jan 25.
Sistan and Baluchestan province, South-East of Iran, has been reported as an endemic area of malaria [Sadrizadeh B. Malaria in the world, in the eastern Mediterranean region and in Iran: Review article. WHO/EMRO Report 2001: 1-13.]. The main objective of this research was to perform rapid and correct diagnoses of malaria infection. Blood specimens were collected from 140 suspected volunteers. The Giemsa-stained slides examination and nested PCR for amplification of the Plasmodium small subunit ribosomal genes (ssrRNA) were utilized. The results demonstrated 118 out of 140 cases (84.3%) positive for malaria parasites, including 60.7%, 20.7% and 2.9% as having Plasmodium vivax (P.v), Plasmodium falciparum (P.f) and mixed infections (P.v+P.f), respectively by microscopy. The nested PCR detected malaria parasites in 134 samples (94.3%), consisting of 51.4% P.v, 12.6% P.f and 29.3% mixed infections. The PCR analysis detected 37 cases of mixed infections more than that of the routine microscopy. These results suggested that there are a considerable number of cases with mixed infections in the study area that mainly remain undiagnosed by microscopy. It is also concluded that the nested PCR is a suitable complement to microscopy for accurate specific diagnosis of malaria species in field.
据报道，伊朗东南部的锡斯坦和俾路支斯坦省是疟疾的流行地区[萨德里扎德·B.《世界、东地中海地区及伊朗的疟疾：综述文章》。世界卫生组织/东地中海区域办事处报告，2001年：1 - 13页。]。本研究的主要目的是对疟疾感染进行快速、准确的诊断。从140名疑似志愿者身上采集了血液样本。采用吉姆萨染色涂片检查和巢式聚合酶链反应（PCR）扩增疟原虫小亚基核糖体基因（ssrRNA）。结果显示，140例中有118例（84.3%）疟原虫检测呈阳性，其中通过显微镜检查，间日疟原虫（P.v）、恶性疟原虫（P.f）和混合感染（P.v + P.f）分别占60.7%、20.7%和2.9%。巢式PCR在134个样本（94.3%）中检测到疟原虫，其中间日疟原虫占51.4%，恶性疟原虫占12.6%，混合感染占29.3%。PCR分析检测出的混合感染病例比常规显微镜检查多37例。这些结果表明，研究区域存在相当数量的混合感染病例，而这些病例主要通过显微镜检查无法确诊。研究还得出结论，巢式PCR是显微镜检查的合适补充手段，可在现场对疟原虫种类进行准确的特异性诊断。
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