伊朗伊斯兰共和国北部间日疟原虫和恶性疟原虫混合感染的分子证据。

Molecular evidence of mixed P. vivax and P. falciparum infections in northern Islamic Republic of Iran.

作者信息

Zakeri S, Mamaghani S, Mehrizi A A, Shahsavari Z, Raeisi A, Arshi S, Dinparast-Djadid N

机构信息

Malaria Research Group, Biotechnology Department, Pasteur Institute of Iran, Tehran, Islamic Republic of Iran.

出版信息

East Mediterr Health J. 2004 May;10(3):336-42.

PMID:16212210

Abstract

This study compared basic microscopy with molecular detection of Plasmodium species. According to thick-film microscopy, 100% of 142 malaria cases in Pars-Abad, Ardebil province, were infected with a single species, P vivax. However, nested polymerase chain reaction (PCR) detected mixed species infections of both P. vivax and P. falciparum in 7.0%. In Mazanderan province, 2/20 blood films were diagnosed with only P. falciparum and 18/20 with only P. vivax. However, nested PCR detected 17/20, 2/20 and 1/20 with P. vivax only, P. falciparum only and mixed species respectively. The unexpected presence of P. falciparum urges prompt investigation and immediate treatment of malaria cases in this region.

摘要

本研究比较了疟原虫种类的基本显微镜检测法和分子检测法。根据厚涂片显微镜检查，阿尔达比勒省帕尔萨巴德的142例疟疾病例中，100%感染的是单一物种，即间日疟原虫。然而，巢式聚合酶链反应（PCR）检测到7.0%的病例同时感染了间日疟原虫和恶性疟原虫。在马赞德兰省，20份血片中2份仅诊断出恶性疟原虫，18份仅诊断出间日疟原虫。然而，巢式PCR检测到20份中有17份仅感染间日疟原虫，2份仅感染恶性疟原虫，1份为混合感染。该地区意外出现恶性疟原虫，促使对疟疾病例进行迅速调查和及时治疗。