Abildgaard U, Norrheim L, Larsen A E, Nesvold A, Sandset P M, Odegaard O R
Department of Medicine with Haematological Research Laboratory, Oslo, Norway.
Haemostasis. 1990;20(4):193-203. doi: 10.1159/000216127.
Three LMW heparins (LMWH), one unfractionated heparin (UH), and international standards of LMWH and UH were compared in three chromogenic substrate (CS) assays and the 'Heptest' clotting assay. With a two-stage CS assay, linear standard curves were obtained in the 0.1-1.0 U/ml range, nearly coinciding for all preparations. With the one-stage CS assays, standard curves were curvilinear and similar for UH and the LMWH groups. In the Heptest assay, standard curves were linear for UH but not for LMWH. Mean recovery of LMWH, added to patients' plasma samples was 70-98% for the four assays. Variation between individual recoveries was much greater with Heptest (coefficient of variation (CV) 35-44%) than with one-stage CS assays (CV 14-21%) or two-stage CS assays (CV 7-8%). For monitoring LMW heparin therapy, CS assays seem preferable to Heptest. The two-stage CS assay had superior accuracy, but the one-stage CS assays were easier to perform.
在三种显色底物（CS）测定法和“Heptest”凝血测定法中，对三种低分子量肝素（LMWH）、一种普通肝素（UH）以及LMWH和UH的国际标准品进行了比较。在两阶段CS测定法中，在0.1 - 1.0 U/ml范围内获得了线性标准曲线，所有制剂的曲线几乎重合。在单阶段CS测定法中，标准曲线呈曲线状，UH组和LMWH组相似。在“Heptest”测定法中，UH的标准曲线是线性的，但LMWH不是。添加到患者血浆样本中的LMWH在四种测定法中的平均回收率为70 - 98%。与单阶段CS测定法（变异系数（CV）14 - 21%）或两阶段CS测定法（CV 7 - 8%）相比，Heptest法中个体回收率之间的差异要大得多（CV 35 - 44%）。对于监测低分子量肝素治疗，CS测定法似乎比“Heptest”更可取。两阶段CS测定法具有更高的准确性，但单阶段CS测定法操作更简便。
