Keatings L, Sinclair J, Wright C, Corbett I P, Watchorn C, Hennessy C, Angus B, Lennard T, Horne C H
Department of Pathology, University of Newcastle-upon-Tyne, UK.
Histopathology. 1990 Sep;17(3):243-7. doi: 10.1111/j.1365-2559.1990.tb00714.x.
Over-expression of the c-erbB-2 oncogene occurs in a proportion of human adenocarcinomas and in breast carcinoma is associated with poorer prognosis. Sections of formalin-fixed, paraffin-embedded tumour tissue from 22 patients with mammary and extramammary Paget's disease have been stained immunohistochemically using a monoclonal antibody (NCL-CB11) raised against a synthetic peptide from the C-terminal end of the predicted sequence of the c-erbB-2 protein product. All 12 cases of mammary Paget's disease showed membrane staining of intra-epidermal cells, indicating c-erbB-2 over-expression. Sections of underlying ductal breast carcinoma were available in nine cases; all nine tumours were c-erbB-2 positive and in eight the in situ component was of comedo or solid type. There was membrane staining of tumour cells in four of the 10 cases of extramammary Paget's disease; staining intensity was generally weaker than that observed in the cases of mammary disease. The possible implications of these findings for the histogenesis of both mammary and extramammary Paget's disease are discussed.
c-erbB-2癌基因在一部分人类腺癌中过度表达，在乳腺癌中与较差的预后相关。使用针对c-erbB-2蛋白产物预测序列C末端合成肽产生的单克隆抗体（NCL-CB11），对22例乳腺和乳腺外佩吉特病患者的福尔马林固定、石蜡包埋肿瘤组织切片进行了免疫组织化学染色。所有12例乳腺佩吉特病病例的表皮内细胞均显示膜染色，表明c-erbB-2过度表达。9例有乳腺导管癌的切片；所有9个肿瘤均为c-erbB-2阳性，其中8个原位成分是粉刺型或实体型。10例乳腺外佩吉特病病例中有4例肿瘤细胞膜染色；染色强度通常比乳腺疾病病例中观察到的弱。讨论了这些发现对乳腺和乳腺外佩吉特病组织发生的可能影响。
