Expression of epidermal-growth-factor receptor in the K562 cell line by transfection. Altered receptor biochemistry.
作者信息
Allen H, Hsuan J, Clark S, Maziarz R, Waterfield M D, Flavell R A, Haley J
机构信息
Immunology Division, National Institute for Medical Research, Mill Hill, London, U.K.
出版信息
Biochem J. 1990 Nov 1;271(3):785-90. doi: 10.1042/bj2710785.
Abstract
The epidermal-growth-factor (EGF) receptor was expressed in the human erythroleukaemic cell line K562 by transfection of the receptor cDNA. EGF-receptor biochemistry appears altered in the K562 transfectants. Autophosphorylation of the K562 receptor is not stimulated substantially by EGF. Tyrosine kinase activity of the receptor is high in the absence of EGF, whereas receptor affinity for EGF is low. K562 cells are shown to lack mRNA for transforming growth factor alpha (TGF alpha). Therefore autocrine stimulation of the K562 receptor, at least by TGF alpha, does not explain the observed receptor biochemistry. The K562 receptor is phosphorylated at a single major site in intact cells, a threonine residue that may be Thr-669. Possible mechanisms of regulation of the EGF receptor in the K562 transfectants are discussed.
摘要
相似文献
1
Expression of epidermal-growth-factor receptor in the K562 cell line by transfection. Altered receptor biochemistry.
Biochem J. 1990 Nov 1;271(3):785-90. doi: 10.1042/bj2710785.
2
A mutant epidermal growth factor receptor with defective protein tyrosine kinase is unable to stimulate proto-oncogene expression and DNA synthesis.一种具有缺陷蛋白酪氨酸激酶的突变型表皮生长因子受体无法刺激原癌基因表达和DNA合成。
Mol Cell Biol. 1987 Dec;7(12):4568-71. doi: 10.1128/mcb.7.12.4568-4571.1987.
3
Transmembrane signalling at the epidermal growth factor receptor. Positive regulation by the C-terminal phosphotyrosine residues.表皮生长因子受体的跨膜信号传导。C 末端磷酸酪氨酸残基的正向调节。
Biochem J. 1991 Jul 15;277 ( Pt 2)(Pt 2):305-11. doi: 10.1042/bj2770305.
4
Polyoma middle T antigen or v-src desensitizes human epidermal growth factor receptor function and interference by a monensin-resistant mutation in mouse Balb/3T3 cells.多瘤病毒中T抗原或v-src使人类表皮生长因子受体功能脱敏，并在小鼠Balb/3T3细胞中受到莫能菌素抗性突变的干扰。
Exp Cell Res. 1992 Dec;203(2):456-65. doi: 10.1016/0014-4827(92)90021-y.
5
The epidermal growth factor receptor and the product of the neu protooncogene are members of a receptor tyrosine phosphorylation cascade.表皮生长因子受体和neu原癌基因的产物是受体酪氨酸磷酸化级联反应的成员。
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6054-7. doi: 10.1073/pnas.87.16.6054.
6
Epidermal growth factor and its receptor.
Mol Cell Endocrinol. 1987 Jun;51(3):169-86. doi: 10.1016/0303-7207(87)90027-x.
7
The EGF/TGF alpha receptor in skin.皮肤中的表皮生长因子/转化生长因子α受体
J Invest Dermatol. 1990 Jun;94(6 Suppl):164S-170S. doi: 10.1111/1523-1747.ep12876141.
8
Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.另一种表皮生长因子受体相关基因的分子克隆与表达
Proc Natl Acad Sci U S A. 1990 Jul;87(13):4905-9. doi: 10.1073/pnas.87.13.4905.
9
Signal transduction by the epidermal growth factor receptor after functional desensitization of the receptor tyrosine protein kinase activity.受体酪氨酸蛋白激酶活性功能脱敏后表皮生长因子受体的信号转导
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6107-11. doi: 10.1073/pnas.87.16.6107.
10
Epidermal growth factor stimulates tyrosine phosphorylation of phospholipase C-II independently of receptor internalization and extracellular calcium.表皮生长因子可刺激磷脂酶C-II的酪氨酸磷酸化，且与受体内化和细胞外钙无关。
Proc Natl Acad Sci U S A. 1989 Mar;86(5):1568-72. doi: 10.1073/pnas.86.5.1568.
引用本文的文献
1
Development and Assessment of 1,5-Diarylpyrazole/Oxime Hybrids Targeting EGFR and JNK-2 as Antiproliferative Agents: A Comprehensive Study through Synthesis, Molecular Docking, and Evaluation.开发和评估 1,5-二芳基吡唑/肟杂合体作为针对 EGFR 和 JNK-2 的抗增殖剂：通过合成、分子对接和评估的综合研究。
Molecules. 2023 Sep 8;28(18):6521. doi: 10.3390/molecules28186521.
2
Lapatinib induces autophagic cell death and differentiation in acute myeloblastic leukemia.拉帕替尼诱导急性髓细胞白血病中的自噬性细胞死亡和分化。
Onco Targets Ther. 2016 Jul 20;9:4453-64. doi: 10.2147/OTT.S105664. eCollection 2016.
3
Optimizing the transduction efficiency of capsid-modified AAV6 serotype vectors in primary human hematopoietic stem cells in vitro and in a xenograft mouse model in vivo.优化体外原代人造血干细胞和体内异种移植小鼠模型中经过衣壳修饰的 AAV6 血清型载体的转导效率。
Cytotherapy. 2013 Aug;15(8):986-98. doi: 10.1016/j.jcyt.2013.04.003.
4
High-efficiency transduction of primary human hematopoietic stem cells and erythroid lineage-restricted expression by optimized AAV6 serotype vectors in vitro and in a murine xenograft model in vivo.优化的 AAV6 血清型载体在体外高效转导原代人造血干细胞和体内小鼠异种移植模型中红系谱系特异性表达。
PLoS One. 2013;8(3):e58757. doi: 10.1371/journal.pone.0058757. Epub 2013 Mar 14.
5
Lapatinib induces autophagy, apoptosis and megakaryocytic differentiation in chronic myelogenous leukemia K562 cells.拉帕替尼诱导慢性髓性白血病 K562 细胞自噬、凋亡和巨核细胞分化。
PLoS One. 2011;6(12):e29014. doi: 10.1371/journal.pone.0029014. Epub 2011 Dec 22.
本文引用的文献
1
Identification of two human beta-tubulin isotypes.两种人类β-微管蛋白亚型的鉴定。
Mol Cell Biol. 1983 May;3(5):854-62. doi: 10.1128/mcb.3.5.854-862.1983.
2
Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.通过电穿孔导入小鼠前B淋巴细胞的人κ免疫球蛋白基因的增强子依赖性表达。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7161-5. doi: 10.1073/pnas.81.22.7161.
3
Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells.人表皮生长因子受体cDNA序列及扩增基因在A431表皮样癌细胞中的异常表达。
Nature. 1984;309(5967):418-25. doi: 10.1038/309418a0.
4
Biosynthesis of the epidermal growth factor receptor in A431 cells.A431细胞中表皮生长因子受体的生物合成。
EMBO J. 1984 Mar;3(3):531-7. doi: 10.1002/j.1460-2075.1984.tb01842.x.
5
Interaction between major histocompatibility complex antigens and epidermal growth factor receptors on human cells.人类细胞上主要组织相容性复合体抗原与表皮生长因子受体之间的相互作用。
J Cell Biol. 1984 Feb;98(2):725-31. doi: 10.1083/jcb.98.2.725.
6
An alteration of the human c-abl protein in K562 leukemia cells unmasks associated tyrosine kinase activity.K562白血病细胞中人类c-abl蛋白的改变揭示了相关的酪氨酸激酶活性。
Cell. 1984 Jul;37(3):1035-42. doi: 10.1016/0092-8674(84)90438-0.
7
A monoclonal antibody to the human epidermal growth factor receptor.一种针对人表皮生长因子受体的单克隆抗体。
J Cell Biochem. 1982;20(2):149-61. doi: 10.1002/jcb.240200207.
8
Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.在A-431细胞膜中鉴定磷酸酪氨酸为表皮生长因子激活的蛋白激酶的产物。
J Biol Chem. 1980 Sep 25;255(18):8363-5.
9
Autophosphorylation sites on the epidermal growth factor receptor.表皮生长因子受体上的自磷酸化位点。
Nature. 1984;311(5985):483-5. doi: 10.1038/311483a0.
10
Autocrine growth factors and cancer.自分泌生长因子与癌症。
Nature. 1985;313(6005):745-7. doi: 10.1038/313745a0.
Zotero 插件