Krummeck G, Rödel G
Labor für Molekulare Biologie und Allgemeine Pathologie, Universität Ulm, München, Federal Republic of Germany.
Curr Genet. 1990 Jul;18(1):13-5. doi: 10.1007/BF00321109.
Biogenesis of functional cytochrome c oxidase in yeast requires the product of the nuclear gene SCO1. Strains deleted for this gene fail to accumulate the mitochondrially-synthesized cytochrome c oxidase subunits I and II, despite the presence of the respective mRNAs. Here we present data which demonstrate that the observed phenotype does not result from a failure to translate the mRNAs, but from a preferential degradation of the newly synthesized subunits. The SCO1 protein is therefore involved in a post-translational step in the accumulation of cytochrome c oxidase subunits I and II. We propose that the SCO1 protein is required for the correct assembly of both subunits into the cytochrome c oxidase complex.
酵母中功能性细胞色素c氧化酶的生物合成需要核基因SCO1的产物。缺失该基因的菌株无法积累线粒体合成的细胞色素c氧化酶亚基I和II，尽管存在相应的mRNA。在此，我们提供的数据表明，观察到的表型并非由于未能翻译mRNA，而是由于新合成的亚基被优先降解。因此，SCO1蛋白参与了细胞色素c氧化酶亚基I和II积累的翻译后步骤。我们认为，SCO1蛋白是两个亚基正确组装到细胞色素c氧化酶复合物中所必需的。
