Département de Biologie, Faculté des Sciences de Gafsa, Campus Universitaire Zarroug-Gafsa 2112, Gafsa, Tunisia.
Nat Prod Res. 2011 Jul;25(12):1195-201. doi: 10.1080/14786419.2010.534097.
In this study, we tested 10 essential oils (EOs) extracted from 10 plants issued from Sned region (Tunisia) to evaluate both their leishmanicidal effects against Leishmania major and L. infantum, and their cytotoxicity against murine macrophage cell line RAW 264.7 (ATCC, TIB-71). The antioxidant activity was also monitored by the DDPH method, while the chemical composition of active EO was assessed by GC-MS analysis. The results showed that the EOs obtained from Thymus hirtus sp. algeriensis (rich on monoterpenoids, especially linalool at 17.62% and camphor at 13.82%) is significantly active against both L. major and L. infantum, whereas Ruta chalepensis EO (rich on 2-undecanone at 84.28%) is only active against L. infantum. Both oil extracts showed low cytotoxicity towards murine macrophages. The characteristic ratios (IC₈₀ Raw264.7 cells/IC₅₀ L. infantum and IC₈₀ Raw264.7 cells/IC₅₀ L. major) were, respectively, 2.7 and 1.57 for T. hirtus sp. algeriensis, and 1.34 and 0.19 for R. chalepensis. However, when measuring the antioxidant effects (DDPH method), the two latter EOs presented a moderate 2,2-diphenyl-2-picrylhydrazyl hydrate scavenging effects compared to EOs from Eucaliptus globulus, Pinus halepensis, Pituranthos tortuosus, Rosmarinus officinalis, Tetraclinis articulata or to BHT.
在这项研究中，我们测试了从突尼斯 Sned 地区（Tunisia）获得的 10 种植物中提取的 10 种精油（EOs），以评估它们对利什曼原虫（Leishmania major 和 L. infantum）的杀利什曼原虫作用以及对小鼠巨噬细胞 RAW 264.7 细胞系（ATCC，TIB-71）的细胞毒性。抗氧化活性也通过 DDPH 法进行监测，而活性 EO 的化学成分则通过 GC-MS 分析进行评估。结果表明，从 Thymus hirtus sp. algeriensis（富含单萜类化合物，尤其是芳樟醇，含量为 17.62%，樟脑含量为 13.82%）中获得的 EO 对 L. major 和 L. infantum 均具有显著的活性，而 Ruta chalepensis EO（富含 2-十一烷酮，含量为 84.28%）仅对 L. infantum 有效。两种油提取物对小鼠巨噬细胞的细胞毒性均较低。特征比（IC₈₀ Raw264.7 细胞/IC₅₀ L. infantum 和 IC₈₀ Raw264.7 细胞/IC₅₀ L. major）分别为 T. hirtus sp. algeriensis 的 2.7 和 1.57，而 R. chalepensis 的 1.34 和 0.19。然而，当测量抗氧化作用（DDPH 法）时，与桉树叶油、油松油、扁柏油、迷迭香油、柏木油或 BHT 相比，后两种 EO 表现出中等的 2,2-二苯基-2-苦肼基水清除作用。
