Matís ohf. Icelandic Food and Biotech R&D, Reykjavík, Iceland.
J Appl Microbiol. 2011 Oct;111(4):971-81. doi: 10.1111/j.1365-2672.2011.05100.x. Epub 2011 Aug 16.
Campylobacter jejuni isolates from various sources in Iceland were genotyped with the aim of assessing the genetic diversity, population structure, source distribution and campylobacter transmission routes to humans.
A collection of 584 Campylobacter isolates were collected from clinical cases, food, animals and environment in Iceland in 1999-2002, during a period of national Campylobacter epidemic in Iceland. All isolates were characterized by pulse field gel electrophoresis (PFGE), and selected subset of 52 isolates representing the diversity of the identified PFGE types was further genotyped using multilocus sequence typing (MLST) and fla-SVR sequencing to gain better insight into the population structure.
The results show a substantial diversity within the Icelandic Campylobacter population. Majority of the human Campylobacter infections originated from domestic chicken and cattle isolates. MLST showed the isolates to be distributed among previously reported and common sequence type complexes in the MLST database.
The genotyping of Campylobacter from various sources has not previously been reported from Iceland, and the results of the study gave a valuable insight into the population structure of Camp. jejuni in Iceland, source distribution and transmission routes to humans. The geographical isolation of Iceland in the north Atlantic provides new information on Campylobacter population dynamics on a global scale.
对来自冰岛不同来源的空肠弯曲菌分离株进行基因分型，旨在评估遗传多样性、种群结构、来源分布以及空肠弯曲菌向人类传播的途径。
1999-2002 年，在冰岛全国性空肠弯曲菌流行期间，从冰岛的临床病例、食品、动物和环境中收集了 584 株空肠弯曲菌分离株。所有分离株均采用脉冲场凝胶电泳（PFGE）进行特征分析，选择具有代表性的 52 株分离株（代表鉴定的 PFGE 型的多样性）进行多位点序列分型（MLST）和 fla-SVR 测序，以更好地了解种群结构。
结果表明，冰岛空肠弯曲菌种群具有很大的多样性。大多数人类空肠弯曲菌感染源自家养鸡和牛分离株。MLST 显示分离株分布在 MLST 数据库中先前报道的和常见的序列型复合体中。
来自冰岛的不同来源的弯曲菌的基因分型以前没有报道过，该研究的结果深入了解了冰岛空肠弯曲菌的种群结构、来源分布和向人类传播的途径。北大西洋的冰岛地理位置为全球范围内弯曲菌种群动态提供了新信息。
