白藜芦醇诱导胰腺癌细胞凋亡。

Resveratrol induces apoptosis in pancreatic cancer cells.

机构信息

Department of Biliary-Pancreatic Surgery, Zhongda Hospital of Southeast University, Nanjing, Jiangsu 210009, China.

出版信息

Chin Med J (Engl). 2011 Jun;124(11):1695-9.

Abstract

BACKGROUND

Pancreatic cancer is one of the most lethal human cancers with a very low survival rate of 5 years. Conventional cancer treatments including surgery, radiation, chemotherapy or combinations of these show little effect on this disease. Several proteins have been proved critical to the development and the progression of pancreatic cancer. The aim of this study was to investigate the effect of resveratrol on apoptosis in pancreatic cancer cells.

METHODS

Several pancreatic cancer cell lines were screened by resveratrol, and its toxicity was tested by normal pancreatic cells. Western blotting was then performed to analyze the molecular mechanism of resveratrol induced apoptosis of pancreatic cancer cell lines.

RESULTS

In the screened pancreatic cancer cell lines, capan-2 and colo357 showed high sensitivity to resveratrol induced apoptosis. Resveratrol exhibited insignificant toxicity to normal pancreatic cells. In resveratrol sensitive cells, capan-2 and colo357, the activation of caspase-3 was detected and showed significant caspase-3 activation upon resveratrol treatment; p53 and p21 were also detected up-regulated upon resveratrol treatment.

CONCLUSION

Resveratrol provides a promising anti-tumor strategy to fight against pancreatic cancer.

摘要

背景

胰腺癌是人类最致命的癌症之一，其 5 年生存率非常低。包括手术、放疗、化疗或这些方法的联合治疗在内的常规癌症治疗对这种疾病几乎没有效果。有几种蛋白质已被证明对胰腺癌的发展和进展至关重要。本研究旨在探讨白藜芦醇对胰腺癌细胞凋亡的影响。

方法

通过白藜芦醇筛选了几种胰腺癌细胞系，并通过正常胰腺细胞测试其毒性。然后通过 Western blot 分析白藜芦醇诱导胰腺癌细胞系凋亡的分子机制。

结果

在筛选出的胰腺癌细胞系中，capan-2 和 colo357 对白藜芦醇诱导的凋亡高度敏感。白藜芦醇对正常胰腺细胞几乎没有毒性。在白藜芦醇敏感的细胞系 capan-2 和 colo357 中，检测到 caspase-3 的激活，并在白藜芦醇处理后显示出显著的 caspase-3 激活；还检测到白藜芦醇处理后 p53 和 p21 的上调。