School of Nutrition and Food Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA.
Pennington Biomedical Research Center, 6400 Perkins Road, Baton Rouge, LA, 70808, USA.
Chem Biol Interact. 2020 Aug 25;327:109142. doi: 10.1016/j.cbi.2020.109142. Epub 2020 Jun 28.
Juglone and thymoquinone are cytotoxic to pancreatic cancer cells. The aim of this study was to investigate, using an analysis of isobolograms, the type and degree of interactions between juglone and thymoquinone on MIA PaCa-2 pancreatic cancer cells. Cell viability was evaluated using the MTT assay. Cell death was determined by flow cytometry. The IC value for juglone and TQ in combination was found to be 24.75 μM, which was higher than juglone or TQ alone. Juglone alone killed Mia Paca-2 cells by ferroptosis. At concentrations where 10, 20 or 50% of cells were affected, there existed a moderate antagonistic relationship between juglone and TQ as indicated by the combination index (CI) value determined by the Compusyn software. At concentrations that affected 75% and 90% of cells, there were nearly an additive effect with CI value of 1.09249 and 0.92391, respectively. Moderate synergism was only seen at concentration where 95% of cells were affected, and the corresponding concentration of juglone and TQ at that combination was 40.90 μM and 511.19 μM, respectively.
胡桃醌和百里醌对胰腺癌细胞具有细胞毒性。本研究旨在通过等辐射图分析，研究胡桃醌和百里醌对 MIA PaCa-2 胰腺癌细胞的相互作用类型和程度。使用 MTT 法评估细胞活力。通过流式细胞术测定细胞死亡。发现胡桃醌和 TQ 联合的 IC 值为 24.75 μM，高于胡桃醌或 TQ 单独使用的值。胡桃醌单独通过铁死亡杀死 Mia Paca-2 细胞。在 10%、20%或 50%的细胞受到影响的浓度下，胡桃醌和 TQ 之间存在中度拮抗关系，这是由 Compusyn 软件确定的组合指数（CI）值表明的。在影响 75%和 90%细胞的浓度下，CI 值分别为 1.09249 和 0.92391，存在近乎相加的作用。仅在影响 95%细胞的浓度下观察到中度协同作用，相应的胡桃醌和 TQ 浓度分别为 40.90 μM 和 511.19 μM。
