State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, China.
Res Microbiol. 2011 Oct;162(8):756-63. doi: 10.1016/j.resmic.2011.06.011. Epub 2011 Jun 22.
Autophagy is a highly conserved process in lower to higher eukaryotic organisms, and occurs in many types of cells as tissues are remodeled during development. In this study, we investigated the functional role of the Trichoderma reesei TrATG5 gene, which encodes an essential protein required for autophagy. TrATG5 is conserved in structure and function in the filamentous fungi and might clearly rescue the pathogenicity function of MgATG5 in Magnaporthe oryzae. Target gene disruption was used to study the functions of TrATG5. It was found that the autophagic process was blocked in the TrATG5 deletion mutant. The mutant was sensitive to nutrient starvation, with abnormal conidiophores and reduced production of conidia. This new evidence might help to elucidate the molecular machinery of autophagy in filamentous fungi.
自噬是一种在从低等到高等真核生物中高度保守的过程，并且在发育过程中组织重塑时发生在许多类型的细胞中。在这项研究中，我们研究了雷氏拟青霉 TrATG5 基因的功能作用，该基因编码自噬所必需的蛋白质。TrATG5 在丝状真菌中的结构和功能是保守的，并且可能清楚地挽救了稻瘟病菌 MgATG5 的致病性功能。靶基因敲除被用于研究 TrATG5 的功能。结果发现，自噬过程在 TrATG5 缺失突变体中被阻断。该突变体对营养饥饿敏感，具有异常的分生孢子梗和减少的分生孢子产生。这一新证据可能有助于阐明丝状真菌中自噬的分子机制。
