State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, Jiliang University, Hangzhou 310018, China.
Int J Mol Sci. 2022 Jul 22;23(15):8104. doi: 10.3390/ijms23158104.
is an important pathogen that causes a devastating disease in rice. It has been reported that the dual-specificity LAMMER kinase is conserved from yeast to animal species and has a variety of functions. However, the functions of the LAMMER kinase have not been reported in . In this study, we identified the unique LAMMER kinase MoKns1 and analyzed its function in . We found that in a deletion mutant, growth and conidiation were primarily decreased, and pathogenicity was almost completely lost. Furthermore, our results found that MoKns1 is involved in autophagy. The Δ mutant was sensitive to rapamycin, and MoKns1 interacted with the autophagy-related protein MoAtg18. Compared with the wild-type strain 70-15, autophagy was significantly enhanced in the Δ mutant. In addition, we also found that MoKns1 regulated DNA damage stress pathways, and the Δ mutant was more sensitive to hydroxyurea (HU) and methyl methanesulfonate (MMS) compared to the wild-type strain 70-15. The expression of genes related to DNA damage stress pathways in the Δ mutant was significantly different from that in the wild-type strain. Our results demonstrate that MoKns1 is an important pathogenic factor in involved in regulating autophagy and DNA damage response pathways, thus affecting virulence. This research on pathogenesis lays a foundation for the prevention and control of .
稻瘟病菌是一种重要的病原体，可导致水稻发生毁灭性病害。据报道，双特异性 LAMMER 激酶在从酵母到动物物种中是保守的，具有多种功能。然而，LAMMER 激酶的功能尚未在 中报道。在本研究中，我们鉴定了独特的 LAMMER 激酶 MoKns1，并分析了其在 中的功能。我们发现，在一个 缺失突变体中，生长和分生孢子形成主要减少，致病性几乎完全丧失。此外，我们的结果发现 MoKns1 参与自噬。Δ突变体对雷帕霉素敏感，MoKns1 与自噬相关蛋白 MoAtg18 相互作用。与野生型菌株 70-15 相比，Δ突变体中的自噬明显增强。此外，我们还发现 MoKns1 调控 DNA 损伤应激途径，与野生型菌株 70-15 相比，Δ突变体对羟基脲 (HU) 和甲基甲磺酸 (MMS) 更敏感。Δ突变体中与 DNA 损伤应激途径相关的基因表达与野生型菌株明显不同。我们的结果表明，MoKns1 是 中一个重要的致病因子，参与调节自噬和 DNA 损伤反应途径，从而影响毒力。本研究为稻瘟病的防治奠定了基础。
