State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, China.
Microbiol Res. 2011 Dec 20;167(1):8-13. doi: 10.1016/j.micres.2011.02.003. Epub 2011 Apr 8.
Magnaporthe oryzae has been used as a model fungal pathogen to study the molecular basis of plant-fungus interactions due to its economic and genetic importance. In this study, we identified a novel gene, Moplaa, which is the homologue of Homo sapiens PLAA encoding a phospholipase A(2)-activating protein. Moplaa is conserved in some eukaryotic organisms by multiple alignment analysis. The function of the Moplaa gene was studied using the gene target replacement method. The Moplaa deletion mutant exhibited retarded growth and conidial germination, reduced conidiation, appressorial turgor pressure and pathogenicity to rice CO-39. Reintroduction of the gene restored defects of the Moplaa deletion mutant.
稻瘟病菌被用作研究植物-真菌相互作用分子基础的模式真菌病原体，因为它具有经济和遗传重要性。在这项研究中，我们鉴定了一个新基因 Moplaa，它是编码磷脂酶 A(2)-激活蛋白的人类 PLAA 的同源物。通过多重比对分析，Moplaa 在一些真核生物中是保守的。使用基因靶向替换方法研究了 Moplaa 基因的功能。Moplaa 缺失突变体表现出生长和分生孢子萌发迟缓、产孢减少、附着胞膨压和对水稻 CO-39 的致病性降低。该基因的回补恢复了 Moplaa 缺失突变体的缺陷。
