Forensic Science Service Ltd, Trident Court 2960 Solihull Parkway, Birmingham Business Park, Birmingham B37 7YN, United Kingdom.
Forensic Sci Int. 2011 Oct 10;212(1-3):210-4. doi: 10.1016/j.forsciint.2011.06.013. Epub 2011 Jul 7.
A method to reliably distinguish menstrual blood from blood in the normal circulation (peripheral blood) would be of considerable use in the forensic analysis and interpretation of evidence in sexual offence investigations. Previous attempts to address this issue have explored microscopy, lactate dehydrogenase isozyme identification, mRNA and miRNA profiling, and identification of the products of fibrinolysis. Here, four assays for D-dimer, a terminal degradation product of fibrinolysis, are evaluated for their specificity and sensitivity in detection of menstrual blood. In addition the effect of exercise, and sample storage upon D-dimer detection was investigated. Comparison of different assays revealed significant differences in results given. Nevertheless, no positive results for D-dimer were obtained using peripheral blood, mixtures of peripheral blood with semen, or peripheral blood taken from donors after moderate exercise. D-dimer was found to be detectable in 100% of menstrual blood samples after 1 week at room temperature and also in samples stored long-term (>3 years) at -20 °C. D-dimer may be an effective, simple to use tool for the presumptive identification of menstrual blood identification.
一种能够可靠地区分月经血和正常循环血（外周血）的方法，将在性犯罪调查中对法医学分析和解释证据有很大的帮助。以前已经尝试通过显微镜检查、乳酸脱氢酶同工酶鉴定、mRNA 和 miRNA 分析以及纤维蛋白溶解产物鉴定来解决这个问题。在这里，我们评估了四种用于检测纤维蛋白溶解终产物 D-二聚体的检测方法的特异性和灵敏度，以检测月经血。此外，还研究了运动和样本储存对 D-二聚体检测的影响。比较不同的检测方法显示出结果存在显著差异。然而，在外周血、外周血与精液的混合物或中等强度运动后的供体外周血中，均未检测到 D-二聚体的阳性结果。在室温下放置 1 周后，100%的月经血样本中可检测到 D-二聚体，在-20°C 下长期储存（>3 年）的样本中也可检测到 D-二聚体。D-二聚体可能是一种有效、简单的工具，可用于月经血的初步鉴定。
