在印度的茶卷叶蛾幼虫中发现了核型多角体病毒的新记录。

New record of nucleopolyhedroviruses in tea looper caterpillars in India.

机构信息

Department of Entomology, Tea Research Association, Tocklai Experimental Station, Jorhat 785 008, Assam, India.

出版信息

J Invertebr Pathol. 2011 Sep;108(1):63-7. doi: 10.1016/j.jip.2011.06.007. Epub 2011 Jun 29.

DOI:10.1016/j.jip.2011.06.007

Abstract

Tea production in North-East India hit a record loss due to the widespread severe outbreak of a mixed brood of three species of looper caterpillar pests of geometrid moths (Lepidoptera) in 2008-2010. In addition to Buzura suppressaria, two newly recorded geometrids, viz., Hyposidra infixaria and Hyposidra talaca have caused widespread severe damage in recent years. In the present study we report the nucleopolyhedroviruses (NPV) isolated from the tea looper caterpillar from North-East India. We identified and characterized the NPV by cloning and sequencing a partial segment of polyhedrin gene of virus infected larvae of B. suppressaria, H. talaca and H. infixaria. A comparison of deduced amino acids of polyhedrin gene among H. talaca, H. infixaria and B. suppressaria showed that same strain was found to infect all the three loopers in India, which show high sequence identity with B. suppressaria Chinese isolates. Based on the polyhedrin sequence homology, it is predicted that a variant of B. suppressaria Chinese isolate of NPV found to infect H. talaca, H. infixaria and B. suppressaria in India.

摘要

2008-2010 年，东北印度的茶叶生产因三种尺蠖毛虫（鳞翅目）混合爆发而遭受严重损失，创下历史纪录。除了 Buzura suppressaria 之外，两种新记录的尺蠖，即 Hyposidra infixaria 和 Hyposidra talaca，近年来也造成了广泛的严重破坏。在本研究中，我们报道了从印度东北部的茶尺蠖中分离到的核多角体病毒（NPV）。我们通过克隆和测序病毒感染的 B. suppressaria、H. talaca 和 H. infixaria 幼虫的多角体基因的部分片段来鉴定和表征 NPV。对 H. talaca、H. infixaria 和 B. suppressaria 多角体基因推导的氨基酸进行比较表明，在印度发现同一种菌株感染所有三种尺蠖，与中国 B. suppressaria 分离株具有高度的序列同一性。基于多角体序列同源性，预测在印度发现的感染 H. talaca、H. infixaria 和 B. suppressaria 的 B. suppressaria 中国分离株的 NPV 变体。