Witz P, Amlaiky N, Plassat J L, Maroteaux L, Borrelli E, Hen R
Laboratoire de Génétique Moléculaire des Eucaryotes dú Centre National de la Recherche Scientifique, Unité 184 de Génie Génétique et de Biologie Moléculaire, Faculté de Médecine, Strasbourg, France.
Proc Natl Acad Sci U S A. 1990 Nov;87(22):8940-4. doi: 10.1073/pnas.87.22.8940.
Using a strategy based on nucleotide sequence homology between genes encoding receptors that interact with guanine nucleotide-binding proteins, we have isolated Drosophila genomic and cDNA clones encoding a functional serotonin receptor (5HT-dro receptor). This protein is expressed predominantly in Drosophila heads and exhibits highest homology with the human 5HT1A receptor. The predicted structure of the 5HT-dro receptor reveals two unusual features: (i) eight putative transmembrane domains instead of the expected seven and (ii) a Gly-Ser repeat that is a potential glycosaminoglycan attachment site. When stably introduced into mouse NIH 3T3 cells, the 5HT-dro receptor activates adenylate cyclase in response to serotonin and is inhibited by serotonin receptor antagonists such as dihydroergocryptine. The 5HT-dro receptor or closely related receptors might be responsible for the serotonin-sensitive cyclase that has been suggested to play a role in learning and modulation of circadian rhythm in a number of invertebrate systems.
利用一种基于与鸟嘌呤核苷酸结合蛋白相互作用的受体编码基因之间核苷酸序列同源性的策略，我们分离出了编码功能性5-羟色胺受体（5HT-dro受体）的果蝇基因组和cDNA克隆。这种蛋白质主要在果蝇头部表达，并且与人类5HT1A受体具有最高的同源性。5HT-dro受体的预测结构显示出两个不同寻常的特征：（i）八个假定的跨膜结构域而非预期的七个；（ii）一个Gly-Ser重复序列，它是一个潜在的糖胺聚糖附着位点。当被稳定导入小鼠NIH 3T3细胞时，5HT-dro受体响应5-羟色胺激活腺苷酸环化酶，并被5-羟色胺受体拮抗剂如双氢麦角隐亭所抑制。5HT-dro受体或与之密切相关的受体可能是5-羟色胺敏感环化酶的原因，该环化酶被认为在许多无脊椎动物系统的学习和昼夜节律调节中发挥作用。
