The Rockefeller University, New York, NY 10021.
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2142-6. doi: 10.1073/pnas.81.7.2142.
Ninety kilobase pairs (kb) of DNA have been isolated from the 3B region of the X chromosome of Drosophila melanogaster. Previous cytogenetic analyses have placed a gene required for rhythmic behavior (per) in this chromosomal interval. Physical characterization of a series of chromosomal rearrangements altering per locus activity indicates that DNA affecting behavioral rhythms is found in a 7.1-kb HindIII fragment. A single 4.5-kb poly(A)(+) RNA is transcribed from this DNA in wild-type pupae and adult flies. The transcript is eliminated by a per mutant that retains some rhythmic activity, but this mutant substitutes two novel transcripts, 11.5 kb and 0.9 kb. It is suggested that the new poly(A)(+) transcripts provide residual per locus activity.
已经从果蝇的 X 染色体 3B 区分离出 90 千碱基对(kb)的 DNA。以前的细胞遗传学分析将一个节律行为所必需的基因(per)定位在这个染色体区间内。一系列改变 per 基因活性的染色体重排的物理特性表明,影响行为节律的 DNA 存在于一个 7.1kb 的 HindIII 片段中。在野生型蛹和成年果蝇中,从这个 DNA 转录出一个单 4.5kb 的 poly(A)(+)RNA。一个保留了一些节律活性的 per 突变体消除了这个转录本,但这个突变体取代了两个新的转录本,11.5kb 和 0.9kb。这表明新的 poly(A)(+)转录本提供了残余的 per 基因活性。