Natural Extracts Unit, The New Zealand Institute for Plant & Food Research Limited, Port Nelson, Nelson 7043, New Zealand.
Anal Biochem. 2011 Oct 15;417(2):289-91. doi: 10.1016/j.ab.2011.06.019. Epub 2011 Jun 22.
An accurate and high-throughput assay for collagen is essential for collagen research and development of collagen products. Hydroxyproline is routinely assayed to provide a measurement for collagen quantification. The time required for sample preparation using acid hydrolysis and neutralization prior to assay is what limits the current method for determining hydroxyproline. This work describes the conditions of alkali hydrolysis that, when combined with the colorimetric assay defined by Woessner, provide a high-throughput, accurate method for the measurement of hydroxyproline.
对于胶原的研究和胶原产品的开发而言，准确且高通量的胶原检测法至关重要。羟脯氨酸的常规检测可提供胶原定量的测量方法。在进行测定之前，使用酸水解和中和进行样品制备所需的时间限制了目前测定羟脯氨酸的方法。本研究描述了碱水解的条件，当与 Woessner 定义的比色测定法结合使用时，提供了一种高通量、准确的羟脯氨酸测量方法。
