School of Civil and Environmental Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
Research and Development Division, H-Plus Eco Ltd, BVC 301, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong, Yuseong-gu, Daejeon 305-333, Republic of Korea.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1199-1203. doi: 10.1099/ijs.0.033308-0. Epub 2011 Jul 8.
Strain MJ31(T), a gram-reaction-positive, aerobic, rod-shaped, non-motile bacterium, was isolated from a sludge sample collected at the Daejeon sewage-disposal plant, in South Korea, and characterized in order to determine its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MJ31(T) belonged to the genus Nocardioides, appearing most closely related to Nocardioides dubius KSL-104(T) (98.6 % sequence similarity), Nocardioides jensenii DSM 20641(T) (97.6 %), Nocardioides daedukensis MDN22(T) (97.2 %) and Nocardioides mesophilus MSL-22(T) (97.0 %). The chemotaxonomic properties of strain MJ31(T) were consistent with those of the genus Nocardioides: MK-8(H(4)) was the predominant menaquinone, iso-C(16 : 0), iso-C(17 : 0) and C(18 : 1)ω9c were the predominant cellular fatty acids, and the cell-wall peptidoglycan was based on LL-2,6-diaminopimelic acid. The genomic DNA G+C content of strain MJ31(T) was 71.2 mol%. Some differential phenotypic properties and low DNA-DNA relatedness values (<28 %) with the type strains of closely related species indicated that strain MJ31(T) represents a novel species, for which the name Nocardioides daejeonensis sp. nov. is proposed. The type strain is MJ31(T) ( = KCTC 19772(T) = JCM 16922(T)).
一株革兰氏反应阳性、需氧、杆状、非运动的细菌菌株 MJ31(T)，从韩国大田污水处理厂的污泥样本中分离得到，为了确定其分类地位对其进行了特征描述。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MJ31(T)属于诺卡氏菌属，与 Nocardioides dubius KSL-104(T)（98.6%序列相似性）、Nocardioides jensenii DSM 20641(T)（97.6%）、Nocardioides daedukensis MDN22(T)（97.2%）和 Nocardioides mesophilus MSL-22(T)（97.0%）最为密切相关。菌株 MJ31(T)的化学分类特征与诺卡氏菌属一致：MK-8(H(4))是主要的类异戊二烯醌，iso-C(16 : 0)、iso-C(17 : 0)和 C(18 : 1)ω9c 是主要的细胞脂肪酸，细胞壁肽聚糖基于 LL-2,6-二氨基庚二酸。菌株 MJ31(T)的基因组 DNA G+C 含量为 71.2 mol%。一些差异表型特征和与密切相关种的模式菌株之间较低的 DNA-DNA 同源性值（<28%）表明，菌株 MJ31(T)代表一个新种，提议将其命名为大田诺卡氏菌（Nocardioides daejeonensis sp. nov.）。模式菌株为 MJ31(T)（=KCTC 19772(T)=JCM 16922(T)）。
