Division of Applied Life Science (BK 21), Plant Molecular Biology & Biotechnology Research Center, Environmental Biotechnology-National Core Research Center, Gyeongsang National University, Jinju 660-701, Republic of Korea.
JGreen Inc., Department of Research & Development, Changnyeong 635-806, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Jan;61(Pt 1):105-109. doi: 10.1099/ijs.0.019919-0. Epub 2010 Feb 19.
A Gram-staining-positive, coccoid to rod-shaped bacterium, designated strain YC6903(T), was isolated from a halophytic plant (Carex scabrifolia Steud.) collected from sand dunes at Namhae Island, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain YC6903(T) grew optimally at 30 °C and at pH 8.0. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YC6903(T) belongs to the genus Nocardioides in the family Nocardioidaceae. Strain YC6903(T) was related most closely to Nocardioides pyridinolyticus OS4(T) (97.0 % 16S rRNA gene sequence similarity), Nocardioides dokdonensis FR1436(T) (96.6 %), Nocardioides aquiterrae GW-9(T) (96.6 %) and Nocardioides hankookensis DS-30(T) (96.6 %). The cell-wall peptidoglycan contained LL-diaminopimelic acid and MK-8(H(4)) was the major respiratory quinone. The mean (±SD) level of DNA-DNA relatedness between strain YC6903(T) and N. pyridinolyticus OS4(T) was 53.5±5.5 %. The predominant cellular fatty acid of strain YC6903(T) was iso-C(16 : 0) (28.9 %). The DNA G+C content was 71.7 mol%. Phenotypic, phylogenetic and chemotaxonomic data indicated that strain YC6903(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides caricicola sp. nov. is proposed. The type strain is YC6903(T) (=KACC 13778(T) =DSM 22177(T)).
一株革兰氏阳性、球形到杆状的细菌，被命名为 YC6903(T) 菌株，从韩国南海岛沙丘上采集的盐生植物（苔草属 scabrifolia Steud.）中分离出来，并用多相方法研究了其分类地位。YC6903(T) 菌株在 30°C 和 pH8.0 下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，YC6903(T) 菌株属于诺卡氏菌属的诺卡氏菌科。YC6903(T) 菌株与 Nocardioides pyridinolyticus OS4(T)（97.0% 16S rRNA 基因序列相似性）、Nocardioides dokdonensis FR1436(T)、Nocardioides aquiterrae GW-9(T) 和 Nocardioides hankookensis DS-30(T)（96.6%）最为密切相关。细胞壁肽聚糖含有 LL-二氨基庚二酸，MK-8(H4) 是主要的呼吸醌。YC6903(T) 菌株与 N. pyridinolyticus OS4(T) 之间的 DNA-DNA 相关度平均值（±SD）为 53.5±5.5%。YC6903(T) 菌株的主要细胞脂肪酸为 iso-C(16：0)（28.9%）。菌株 YC6903(T) 的 DNA G+C 含量为 71.7mol%。表型、系统发育和化学分类学数据表明，YC6903(T) 菌株代表了诺卡氏菌属的一个新种，命名为 Caricicola nocardioides sp. nov.。模式菌株为 YC6903(T)=KACC 13778(T)=DSM 22177(T)。
