通过一种新型锚定蛋白将活性β-葡萄糖苷酶展示在细胞表面，从而构建能够利用纤维二糖的大肠杆菌菌株。
Creation of a cellooligosaccharide-assimilating Escherichia coli strain by displaying active beta-glucosidase on the cell surface via a novel anchor protein.
机构信息
Organization of Advanced Science and Technology, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
出版信息
Appl Environ Microbiol. 2011 Sep;77(17):6265-70. doi: 10.1128/AEM.00459-11. Epub 2011 Jul 8.
Abstract
We demonstrated direct assimilation of cellooligosaccharide using Escherichia coli displaying beta-glucosidase (BGL). BGL from Thermobifida fusca YX (Tfu0937) was displayed on the E. coli cell surface using a novel anchor protein named Blc. This strain was grown successfully on 0.2% cellobiose, and the optical density at 600 nm (OD(600)) was 1.05 after 20 h.
摘要
我们利用展示β-葡萄糖苷酶（BGL）的大肠杆菌展示了纤维二糖寡糖的直接同化。来自嗜热纤维芽孢杆菌 YX（Tfu0937）的 BGL 使用一种名为 Blc 的新型锚定蛋白展示在大肠杆菌细胞表面。该菌株在 0.2%的纤维二糖上成功生长，20 小时后 600nm 处的光密度（OD（600））为 1.05。
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