Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Trends Biotechnol. 2011 Feb;29(2):79-86. doi: 10.1016/j.tibtech.2010.11.003. Epub 2010 Dec 9.
Bacterial surface display entails the presentation of recombinant proteins or peptides on the surface of bacterial cells. Escherichia coli is the most frequently used bacterial host for surface display and, as such, a variety of E. coli display systems have been described that primarily promote the surface exposure of peptides and small proteins. By contrast, display systems based on autotransporter proteins (ATs) and ice nucleation protein (INP) are excellent systems for the display of large and complex proteins, and are therefore of considerable biotechnological relevance. Here, we review recent advances in AT and INP-mediated display and their biotechnological applications. Additionally, we discuss several promising alternative display methods, as well as novel bacterial host organisms.
细菌表面展示技术是指将重组蛋白或肽展示在细菌细胞表面。大肠杆菌是最常用于表面展示的细菌宿主，因此，已经描述了多种大肠杆菌展示系统，这些系统主要促进肽和小蛋白的表面暴露。相比之下，基于自动转运蛋白 (AT) 和冰核蛋白 (INP) 的展示系统是展示大型和复杂蛋白的优秀系统，因此具有相当大的生物技术相关性。在这里，我们回顾了 AT 和 INP 介导的展示技术的最新进展及其生物技术应用。此外，我们还讨论了几种有前途的替代展示方法以及新型细菌宿主。
