TWIST 和 p-Akt 在正常口腔上皮、口腔发育不良和口腔鳞状细胞癌中的免疫表达。
TWIST and p-Akt immunoexpression in normal oral epithelium, oral dysplasia and in oral squamous cell carcinoma.
机构信息
Department of Oral Pathology, Dental School, University of São Paulo, São Paulo, São Paulo, Brazil.
出版信息
Med Oral Patol Oral Cir Bucal. 2012 Jan 1;17(1):e29-34. doi: 10.4317/medoral.17344.
OBJECTIVES
The aim of this study was to evaluate the immunoexpression of TWIST and p-Akt proteins in oral leukoplakia (OL) and oral squamous cell carcinoma (OSCC), correlating their expressions with the histological features of the lesions.
STUDY DESIGN
Immunohistochemical studies were carried out on 10 normal oral epithelium, 30 OL and 20 OSCC formalin-fixed, paraffin-embedded tissue samples. Immunoperoxidase reactions for TWIST and p-Akt proteins were applied on the specimens and the positivity of the reactions was calculated for 1000 epithelial cells.
RESULTS
Kruskal-Wallis and Dunn's post tests revealed a significant difference in TWIST and p- Akt immunoexpression among normal oral mucosa, OL and OSCC. In addition, a significant positive correlation was found between TWIST and p-Akt expressions according to the Pearson's correlation test.
CONCLUSIONS
The results obtained in the current study suggest that TWIST and p-Akt may participate of the multi-step process of oral carcinogenesis since its early stages.
目的
本研究旨在评估 TWIST 和 p-Akt 蛋白在口腔白斑（OL）和口腔鳞状细胞癌（OSCC）中的免疫表达，将其表达与病变的组织学特征相关联。
研究设计
对 10 例正常口腔上皮、30 例 OL 和 20 例 OSCC 的福尔马林固定、石蜡包埋组织样本进行了免疫组织化学研究。应用免疫过氧化物酶反应检测 TWIST 和 p-Akt 蛋白在标本中的表达，并计算 1000 个上皮细胞的反应阳性率。
结果
Kruskal-Wallis 和 Dunn 事后检验显示，TWIST 和 p-Akt 的免疫表达在正常口腔黏膜、OL 和 OSCC 之间存在显著差异。此外，根据 Pearson 相关检验，发现 TWIST 和 p-Akt 的表达之间存在显著的正相关。
结论
本研究结果表明，TWIST 和 p-Akt 可能参与了口腔癌变的多步过程，从早期阶段就开始了。
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