Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, 71037-11351, Shiraz, Iran.
Med Oral Patol Oral Cir Bucal. 2011 Nov 1;16(7):e1031-5. doi: 10.4317/medoral.17174.
To test the antimicrobial sensitivity of Porphyromonas gingivalis to a panel of eight orally administrable antibiotics in chronic periodontal diseases and to evaluate factors associated with periodontitis in adult patients.
A total of fifty strains of P. gingivalis were isolated from one hundred and twenty adult patients with chronic perio-dontitis. Identification of bacteria was carried out by anaerobic culture and biochemical tests. Selected colonies of P. gingivalis were used to evaluate the antibacterial activities of penicillin, metronidazole, amoxicillin, amoxicillin/clavulanic acid, clindamycin, doxy-cycline, ciprofloxacin and azithromycin.
Most of the patients were female, age ranging between 40 to 50 years. Majority of the patients frequently had scaling and depths of periodontal pockets in infected teeth were 5-8 mm and most of them had hemorrhage during sampling. Susceptibility testing revealed a sensitivity of 100% of P. gingivalis to azithromycin, doxycycline and amoxicillin/clavulanic acid but lower susceptibilities were found for the rest of antibiotic agents evaluated.
Frequent scaling in women aged between 40-50 years had positive correlation with chronic periodontitis. The application of antibiotics in conjuction with mechanical debridation, may reflect in the level of resistance of P. gingivalis in patients with chronic periodontal infections. This could suggest periodical antibiotic susceptibility testing is necessary to determine the efficacy of antimicrobial agents if the perfect curing of chronic periodontal diseases after mechanical debridation is meant. Further clinical studies are required to confirm the in vitro results. The only limitation in this study was identification of bacteria to species rather than subspecies level.
检测慢性牙周炎患者牙龈卟啉单胞菌对 8 种口服抗生素的抗菌敏感性，并评估与成人牙周炎相关的因素。
从 120 名慢性牙周炎成年患者中分离出 50 株牙龈卟啉单胞菌。通过厌氧培养和生化试验进行细菌鉴定。选用牙龈卟啉单胞菌的选定菌落，评估青霉素、甲硝唑、阿莫西林、阿莫西林/克拉维酸、克林霉素、多西环素、环丙沙星和阿奇霉素的抗菌活性。
大多数患者为女性，年龄在 40 至 50 岁之间。大多数患者经常有牙石，感染牙齿的牙周袋深度为 5-8mm，大部分在取样时都有出血。药敏试验显示，牙龈卟啉单胞菌对阿奇霉素、多西环素和阿莫西林/克拉维酸的敏感性为 100%，而对其余评估抗生素的敏感性较低。
40-50 岁女性经常进行牙石清除与慢性牙周炎有正相关关系。抗生素与机械清创术联合应用，可能反映出慢性牙周感染患者中牙龈卟啉单胞菌的耐药水平。这表明如果要达到机械清创术后慢性牙周病的完美治愈，有必要定期进行抗生素药敏试验，以确定抗菌药物的疗效。需要进一步的临床研究来证实体外结果。本研究的唯一局限性是将细菌鉴定到种而非亚种水平。
