Section of Periodontology, Faculty of Odontology, University Complutense, Ciudad Universitaria, Plaza Ramón y Cajal s/n, 28040, Madrid, Spain.
EREB (Oral Ecosystem) Research Group, Faculty of Medicine Dentistry, Mohammed V University, Rabat, Morocco.
Clin Oral Investig. 2019 Mar;23(3):1161-1170. doi: 10.1007/s00784-018-2539-2. Epub 2018 Jul 2.
Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis have been frequently isolated in periodontitis patients in Morocco. Its persistence after the subgingival debridement of the biofilm has been correlated with worse clinical outcomes. The aim of this study was to evaluate the antimicrobial susceptibilities of A. actinomycetemcomitans and P. gingivalis, to amoxicillin, amoxicillin plus clavulanate, metronidazole, and azithromycin. In addition, microbiological profiles of patients harbouring A. actinomycetemcomitans, P. gingivalis, or both were compared.
In 45 consecutive periodontitis Moroccan patients, subgingival samples were taken and processed by culture. Twenty-four A. actinomycetemcomitans and 30 P. gingivalis colonies were isolated (54 strains) and susceptibility tests, using the epsilometric method, were run for amoxicillin, amoxicillin plus clavulanate, metronidazole, and azithromycin. Minimum inhibitory concentrations for 50 (MIC50) and 90% (MIC90) of the organisms were calculated.
The prevalence of A. actinomycetemcomitans and P. gingivalis was 79.5 and 84.4%, respectively. A. actinomycetemcomitans showed susceptibility to amoxicillin, amoxicillin plus clavulanate, while 28% of the isolated strains were resistant to azithromycin and 61.7% towards metronidazole. No P. gingivalis resistance towards amoxicillin, amoxicillin plus clavulanate, metronidazole, and azithromycin was found.
A. actinomycetemcomitans and P. gingivalis were frequently detected in Moroccan patients with periodontitis, while antimicrobial resistance was only detected for A. actinomycetemcomitans to metronidazole and azithromycin.
A. actinomycetemcomitans resistance against some antimicrobials in periodontitis patients in Morocco can influence the selection of the therapeutic approaches.
在摩洛哥的牙周炎患者中，经常分离到伴放线放线杆菌和牙龈卟啉单胞菌。生物膜龈下清创后其持续存在与更差的临床结局相关。本研究旨在评估伴放线放线杆菌和牙龈卟啉单胞菌对阿莫西林、阿莫西林加克拉维酸、甲硝唑和阿奇霉素的药敏情况。此外，还比较了携带伴放线放线杆菌、牙龈卟啉单胞菌或两者的患者的微生物学特征。
在 45 例连续的摩洛哥牙周炎患者中，采集龈下样本并通过培养进行处理。分离出 24 株伴放线放线杆菌和 30 株牙龈卟啉单胞菌（54 株菌），并采用比浊法进行阿莫西林、阿莫西林加克拉维酸、甲硝唑和阿奇霉素的药敏试验。计算 50%（MIC50）和 90%（MIC90）的最小抑菌浓度。
伴放线放线杆菌和牙龈卟啉单胞菌的流行率分别为 79.5%和 84.4%。伴放线放线杆菌对阿莫西林、阿莫西林加克拉维酸敏感，而 28%的分离株对阿奇霉素耐药，61.7%对甲硝唑耐药。未发现牙龈卟啉单胞菌对阿莫西林、阿莫西林加克拉维酸、甲硝唑和阿奇霉素耐药。
伴放线放线杆菌和牙龈卟啉单胞菌在摩洛哥牙周炎患者中经常被检测到，而只有伴放线放线杆菌对甲硝唑和阿奇霉素表现出耐药性。
在摩洛哥的牙周炎患者中，伴放线放线杆菌对某些抗生素的耐药性可能会影响治疗方法的选择。
