Department of Physics, The Chinese University of Hong Kong, Shatin, New Territory, Hong Kong.
Nanoscale. 2011 Aug;3(8):3291-9. doi: 10.1039/c1nr10499c. Epub 2011 Jul 11.
A systematic study on the interaction of silica nanoparticles (NPs) with human cells has been carried out in the present work. Endocytosis and exocytosis are identified as major pathways for NPs entering, and exiting the cells, respectively. Most of the NPs are found to be enclosed in membrane bounded organelles, which are fairly stable (against rupture) as very few NPs are released into the cytoplasm. The nanoparticle-cell interaction is a dynamic process, and the amount of NPs inside the cells is affected by both the amount and morphology (degree of aggregation) of NPs in the medium. These interaction characteristics determine the low cytotoxicity of SiO(2) NPs at low feeding concentration.
本工作系统研究了二氧化硅纳米颗粒（NPs）与人细胞的相互作用。内吞作用和外排作用分别被确定为 NPs 进入和离开细胞的主要途径。大多数 NPs 被发现包裹在膜结合细胞器中，这些细胞器非常稳定（不易破裂），因为很少有 NPs 释放到细胞质中。纳米颗粒与细胞的相互作用是一个动态过程，细胞内的 NPs 数量受介质中 NPs 的数量和形态（聚集程度）的影响。这些相互作用特征决定了低浓度喂养时 SiO(2) NPs 的低细胞毒性。
