National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai, China.
Mol Cell Biochem. 2011 Dec;358(1-2):317-23. doi: 10.1007/s11010-011-0982-3. Epub 2011 Jul 9.
Dendritic cells (DC) and myeloid-derived suppressor cells (MDSC) are important cells involved in immune response. DC can be generated from mouse bone marrow (BM) in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4. Recent studies have revealed that combined treatment of bone marrow MDSC with LPS plus IFN-γ inhibited the DC development but enhanced MDSC functions, such as NO release and T cell suppression. In our study, bone marrow precursor cells cultures in GM-CSF and IL-4 were treated with poly(I:C) through the culture, Gr1(+)CD11b(+) cells with MDSC functions, such as NO release and T cell suppression were accumulated in the culture system. Then the similar phenomenon was observed in the vesicular stomatitis virus infection in vivo. In conclusion, we demonstrated that the bone marrow precursor cells in the presence of GM-CSF and IL-4 can differentiate into MDSC, which is dependent on the dynamic of interaction with poly(I:C).
树突状细胞 (DC) 和髓系来源的抑制细胞 (MDSC) 是参与免疫反应的重要细胞。在粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 和白细胞介素-4 (IL-4) 的存在下，可从小鼠骨髓 (BM) 中产生 DC。最近的研究表明，用 LPS 和 IFN-γ 联合处理骨髓 MDSC 可抑制 DC 的发育，但增强 MDSC 的功能，如 NO 释放和 T 细胞抑制。在我们的研究中，通过培养用多聚 (I:C) 处理在 GM-CSF 和 IL-4 中培养的骨髓前体细胞，在培养系统中积累了具有 NO 释放和 T 细胞抑制等 MDSC 功能的 Gr1(+)CD11b(+) 细胞。然后在体内水疱性口炎病毒感染中观察到类似的现象。总之，我们证明了在 GM-CSF 和 IL-4 存在下的骨髓前体细胞可分化为 MDSC，这依赖于与多聚 (I:C) 的动态相互作用。
