Matowicka-Karna Joanna, Kralisz Maciej, Kemona Halina
Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, Poland.
Folia Histochem Cytobiol. 2011;49(2):280-4. doi: 10.5603/fhc.2011.0039.
The current study aims to determine the involvement of cellular responses in combating Giardia intestinalis invasion. The study group consisted of 44 women and 18 men, aged 18-72 years, infected with G. intestinalis. The diagnosis was established based on laboratory investigations (examination of stool, choloscopy, GSA-65). Blood for analysis was collected before antiparasitic treatment and two weeks after treatment termination. The control group consisted of 22 women and 18 men aged 20-45 years. The serum concentrations of IL-5, IL-6, IL-13, TNF, IFN-g were assayed using a set of Quantikine human. The concentrations of NO in the serum were determined using a set of Total Nitric Oxide Assay. Patients infected with G. intestinalis showed a statistically significant increase in the levels of NO, IFN-g and IL-13. Even the antiparasitic treatment applied did not reduce the levels of these parameters and only caused a rise in IL-6. Our study showed a lack of acute inflammatory state in the course of G. intestinalis infection.
本研究旨在确定细胞反应在对抗肠道贾第虫感染中的作用。研究组由44名女性和18名男性组成，年龄在18至72岁之间，均感染了肠道贾第虫。诊断基于实验室检查（粪便检查、胆汁镜检查、GSA - 65）。在进行抗寄生虫治疗前及治疗结束两周后采集血液进行分析。对照组由22名女性和18名男性组成，年龄在20至45岁之间。使用一套Quantikine人用试剂盒检测血清中IL - 5、IL - 6、IL - 13、TNF、IFN - g的浓度。使用一套总一氧化氮检测试剂盒测定血清中NO的浓度。感染肠道贾第虫的患者血清中NO、IFN - g和IL - 13水平有统计学意义的升高。即使进行了抗寄生虫治疗，这些参数的水平也没有降低，只是导致IL - 6升高。我们的研究表明，在肠道贾第虫感染过程中不存在急性炎症状态。
