Kaunas University of Technology, Radvilenu pl. 19, LT-50254 Kaunas, Lithuania.
J Sci Food Agric. 2012 Jan 15;92(1):84-91. doi: 10.1002/jsfa.4545. Epub 2011 Jul 11.
The efficiency of bioethanol production from wheat biomass is related to the quality of end products as well as to safety criteria of co-products such as distiller's dried grains with solubles (DDGS). The inclusion of a new biocatalyst for non-starch polysaccharide degradation in fermentation processes could be one of the solutions. The objective of this study was to evaluate the influence of β-xylanases in combination with traditional amylolytic enzymes on the efficiency of bioethanol production and DON detoxification during fermentation of Fusarium-contaminated wheat biomass with high concentration of deoxynivalenol (DON; 3.95 mg kg(-1)).
The results showed that the negative effect of Fusarium spp. on yield and quality of bioethanol could be eliminated by the application of Trichoderma reesei xylanase in combination with amylolytic enzymes. This technological solution allowed to increase the concentration of ethanol in the fermented wort by 35.3% and to improve the quality of bioethanol by decreasing the concentrations of methanol, methyl acetate, isoamyl and isobutyl alcohols. Mass balance calculations showed that DDGS was the main source of DON contamination, comprising 74% of toxin found in wheat biomass. By using new enzyme combination for wheat biomass saccharification, a higher level of detoxification (41%) of DON was achieved during the fermentation process.
The addition of Trichoderma reesei xylanase played a positive role in bioethanol production from Fusarium-contaminated wheat biomass, indicating that the yeast-growing medium was enriched during the enzymatic treatment.
从小麦生物质生产生物乙醇的效率与终产物的质量以及酒糟（DDGS）等副产物的安全标准有关。在发酵过程中添加一种新的非淀粉多糖降解生物催化剂可能是解决方案之一。本研究的目的是评估β-木聚糖酶与传统淀粉分解酶联合使用对含有高浓度脱氧雪腐镰刀菌烯醇（DON；3.95mg/kg）的污染小麦生物质发酵过程中生物乙醇生产效率和 DON 解毒的影响。
结果表明，通过应用里氏木霉木聚糖酶与淀粉分解酶联合使用，可以消除镰刀菌属对生物乙醇产量和质量的负面影响。该技术解决方案可将发酵麦汁中的乙醇浓度提高 35.3%，并通过降低甲醇、甲基乙酸酯、异戊醇和异丁醇的浓度来提高生物乙醇的质量。物料平衡计算表明，DDGS 是 DON 污染的主要来源，占小麦生物质中发现毒素的 74%。通过使用新的酶组合对小麦生物质进行糖化，可以在发酵过程中实现更高水平的 DON 解毒（41%）。
里氏木霉木聚糖酶的添加对污染小麦生物质生产生物乙醇起到了积极作用，表明在酶处理过程中酵母生长培养基得到了富集。
