Institute of Biotechnology, Hurlingham, Argentina.
BMC Vet Res. 2011 Jul 12;7:37. doi: 10.1186/1746-6148-7-37.
In many regions of the world, wild mammals act as reservoir of Mycobacterium bovis, a situation that prevents the eradication of bovine tuberculosis. In order to observe whether a strain isolated from a wild boar, previously tested as highly virulent in a mice model, is also virulent in cattle, we performed cattle experimental inoculation with this strain
Groups of Friesian calves were either infected with the wild boar strain M. bovis 04-303 or with the bovine strain NCTC10772 as a control. We found that antigen-specific IFN-γ release in whole blood samples occurred earlier in animals infected with M. bovis 04-303. Both M. bovis strains resulted in a positive skin test, with animals infected with the wild boar isolate showing a stronger response. These results and the presence of more severe organ lesions, with granuloma and pneumonic areas in cattle demonstrate that the wild boar isolate is more virulent than the NCTC10772 strain. Additionally, we tested the infectivity of the M. bovis strains in guinea pigs and found that M. bovis 04-303 had the highest pathogenicity.
M. bovis strains isolated from wild boars may be pathogenic for cattle, producing TB lesions.
在世界许多地区，野生动物是牛分枝杆菌（Mycobacterium bovis）的储存宿主，这种情况阻碍了牛结核病的根除。为了观察从野猪中分离出来的一株先前在小鼠模型中被测试为高毒力的菌株是否也对牛具有毒力，我们用该菌株对牛进行了实验性接种。
弗里斯兰小牛组要么感染野猪株 M. bovis 04-303，要么感染牛株 NCTC10772 作为对照。我们发现，在感染 M. bovis 04-303 的动物中，全血样本中抗原特异性 IFN-γ 的释放更早发生。两种 M. bovis 菌株均导致皮肤试验阳性，野猪分离株感染的动物反应更强。这些结果以及更严重的器官病变的存在，包括牛的肉芽肿和肺炎区域，表明野猪分离株比 NCTC10772 株更具毒力。此外，我们测试了豚鼠中 M. bovis 菌株的感染性，发现 M. bovis 04-303 具有最高的致病性。
从野猪中分离出的 M. bovis 菌株可能对牛具有致病性，导致结核病病变。
