Dai R S, Liu G H, Song H Q, Lin R Q, Yuan Z G, Li M W, Huang S Y, Liu W, Zhu X Q
College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province 410128, PR China.
J Helminthol. 2012 Jun;86(2):245-51. doi: 10.1017/S0022149X11000319. Epub 2011 Jul 12.
Sequence variability in two mitochondrial DNA (mtDNA) regions, namely cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 4 (nad4), and internal transcribed spacer (ITS) of rDNA among and within three cestodes, Spirometra erinaceieuropaei, Taenia multiceps and Taenia hydatigena, from different geographical origins in China was examined. A portion of the cox1 (pcox1), nad4 genes (pnad4) and the ITS (ITS1+5.8S rDNA+ITS2) were amplified separately from individual cestodes by polymerase chain reaction (PCR). Representative amplicons were subjected to sequencing in order to estimate sequence variability. While the intra-specific sequence variations within each of the tapeworm species were 0-0.7% for pcox1, 0-1.7% for pnad4 and 0.1-3.6% for ITS, the inter-specific sequence differences were significantly higher, being 12.1-17.6%, 18.7-26.2% and 31-75.5% for pcox1, pnad4 and ITS, respectively. Phylogenetic analyses based on the pcox1 sequence data revealed that T. multiceps and T. hydatigena were more closely related to the other members of the Taenia genus, and S. erinaceieuropaei was more closely related to the other members of the Spirometra genus. These findings demonstrated clearly the usefulness of mtDNA and rDNA sequences for population genetic studies of these cestodes of socio-economic importance.
对来自中国不同地理区域的三种绦虫，即曼氏迭宫绦虫、多头带绦虫和有钩绦虫，其两个线粒体DNA（mtDNA）区域，即细胞色素c氧化酶亚基1（cox1）和NADH脱氢酶亚基4（nad4），以及核糖体DNA的内部转录间隔区（ITS）的序列变异性进行了研究。通过聚合酶链反应（PCR）从单个绦虫中分别扩增出一部分cox1（pcox1）、nad4基因（pnad4）和ITS（ITS1 + 5.8S rDNA + ITS2）。对代表性扩增子进行测序以估计序列变异性。虽然每种绦虫物种内的种内序列变异对于pcox1为0 - 0.7%，对于pnad4为0 - 1.7%，对于ITS为0.1 - 3.6%，但种间序列差异明显更高，pcox1、pnad4和ITS分别为12.1 - 17.6%、18.7 - 26.2%和31 - 75.5%。基于pcox1序列数据的系统发育分析表明，多头带绦虫和有钩绦虫与带绦虫属的其他成员关系更密切，而曼氏迭宫绦虫与迭宫绦虫属的其他成员关系更密切。这些发现清楚地证明了mtDNA和rDNA序列对于这些具有社会经济重要性的绦虫的群体遗传学研究的有用性。
