Department of Chemistry, Temple University, Philadelphia, PA 19122, USA.
Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12217-22. doi: 10.1073/pnas.1105673108. Epub 2011 Jul 11.
Femtosecond laser vaporization-based mass spectrometry can be used to measure protein conformation in vitro at atmospheric pressure. Cytochrome c and lysozyme are vaporized from the condensed phase into the gas phase intact when exposed to an intense (10(13) W/cm(2)), nonresonant (800 nm), ultrafast (75 fs) laser pulse. Electrospray postionization time-of-flight mass spectrometry reveals that the vaporized protein maintains the solution-phase conformation through measurement of the charge-state distribution and the collision-induced dissociation channels.
飞秒激光汽化质谱法可用于在大气压下测量体外蛋白质构象。当暴露于强(10(13) W/cm(2))、非共振(800nm)、超快(75fs)激光脉冲时,细胞色素 c 和溶菌酶从凝聚相完整地汽化成气相。电喷雾正离子化飞行时间质谱法通过测量电荷态分布和碰撞诱导解离通道,表明汽化蛋白质保持溶液相构象。