非共振飞秒激光汽化水溶液中的蛋白质可保持其折叠结构。
Nonresonant femtosecond laser vaporization of aqueous protein preserves folded structure.
机构信息
Department of Chemistry, Temple University, Philadelphia, PA 19122, USA.
出版信息
Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12217-22. doi: 10.1073/pnas.1105673108. Epub 2011 Jul 11.
Abstract
Femtosecond laser vaporization-based mass spectrometry can be used to measure protein conformation in vitro at atmospheric pressure. Cytochrome c and lysozyme are vaporized from the condensed phase into the gas phase intact when exposed to an intense (10(13) W/cm(2)), nonresonant (800 nm), ultrafast (75 fs) laser pulse. Electrospray postionization time-of-flight mass spectrometry reveals that the vaporized protein maintains the solution-phase conformation through measurement of the charge-state distribution and the collision-induced dissociation channels.
摘要
飞秒激光汽化质谱法可用于在大气压下测量体外蛋白质构象。当暴露于强（10(13) W/cm(2)）、非共振（800nm）、超快（75fs）激光脉冲时，细胞色素 c 和溶菌酶从凝聚相完整地汽化成气相。电喷雾正离子化飞行时间质谱法通过测量电荷态分布和碰撞诱导解离通道，表明汽化蛋白质保持溶液相构象。
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本文引用的文献
1
Ion formation from charged droplets: Roles of geometry, energy, and time.带电液滴的离子形成：几何形状、能量和时间的作用。
J Am Soc Mass Spectrom. 1993 Jul;4(7):524-35. doi: 10.1016/1044-0305(93)85014-O.
2
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J Am Soc Mass Spectrom. 2011 Apr;22(4):762-72. doi: 10.1007/s13361-010-0066-8. Epub 2011 Feb 8.
3
Nonresonant femtosecond laser vaporization with electrospray postionization for ex vivo plant tissue typing using compressive linear classification.使用压缩线性分类的非共振飞秒激光蒸发与喷雾电喷雾离子化对植物组织进行离体组织分型。
Anal Chem. 2011 Mar 15;83(6):2145-51. doi: 10.1021/ac102978f. Epub 2011 Feb 25.
4
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5
An electrospray/inductively coupled plasma dual-source time-of-flight mass spectrometer for rapid metallomic and speciation analysis. Part 1. Molecular channel characterization.一种用于快速金属组学和形态分析的电喷雾/电感耦合等离子体双源飞行时间质谱仪。第 1 部分。分子通道特性。
Metallomics. 2010 Apr;2(4):271-9. doi: 10.1039/b915782d. Epub 2010 Feb 1.
6
Identification of explosives and explosive formulations using laser electrospray mass spectrometry.利用激光电喷雾质谱鉴定爆炸物和爆炸配方。
Rapid Commun Mass Spectrom. 2010 Jun 15;24(11):1659-64. doi: 10.1002/rcm.4566.
7
Analysis of pharmaceutical compounds from glass, fabric, steel, and wood surfaces at atmospheric pressure using spatially resolved, nonresonant femtosecond laser vaporization electrospray mass spectrometry.使用空间分辨、非共振飞秒激光汽化电喷雾质谱法分析常压下玻璃、织物、钢和木材表面的药物化合物。
Anal Chem. 2010 Apr 15;82(8):3231-8. doi: 10.1021/ac902880q.
8
Effects of matrix, electrospray solution, and laser light on the desorption and ionization mechanisms in electrospray-assisted laser desorption ionization mass spectrometry.基质、电喷雾溶液和激光对电喷雾辅助激光解吸电离质谱中解吸和电离机制的影响。
Analyst. 2010 Apr;135(4):759-66. doi: 10.1039/b923239g. Epub 2010 Feb 2.
9
Mass spectrometry of intact neutral macromolecules using intense non-resonant femtosecond laser vaporization with electrospray post-ionization.使用强非共振飞秒激光汽化结合电喷雾后电离对完整中性大分子进行质谱分析。
Rapid Commun Mass Spectrom. 2009 Oct;23(19):3151-7. doi: 10.1002/rcm.4226.
10
Phase matching of high harmonic generation in the soft and hard X-ray regions of the spectrum.光谱软X射线和硬X射线区域高次谐波产生的相位匹配
Proc Natl Acad Sci U S A. 2009 Jun 30;106(26):10516-21. doi: 10.1073/pnas.0903748106. Epub 2009 Jun 18.
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