Taylor A H, Ang V T, Jenkins J S, Silverlight J J, Coombes R C, Luqmani Y A
Division of Biochemical Medicine, St. George's Hospital Medical School, London, England.
Cancer Res. 1990 Dec 15;50(24):7882-6.
The arginine vasopressin and oxytocin content of normal and cancerous human breast tissue were measured using radioimmunoassay. Both peptides were present in amounts greater than that found in the circulation, but no difference between normal and malignant tissues was found. Binding of [3H]oxytocin and [3H]vasopressin were characterized in human breast carcinoma cells (MCF7 cells). Binding of both hormones to MCF7 cells was specific and saturable, the vasopressin receptor found to be of the V1 subtype. Scatchard analyses of the data were linear, indicating a single high affinity, low capacity binding site for each hormone (vasopressin: KD = 47.4 +/- 1.6 nmol/liter, Bmax = 27,300 +/- 6,500 sites/cell; oxytocin: KD = 51.3 +/- 0.4 nmol/liter, Bmax = 87,000 +/- 4,000 sites/cell). The effects of vasopressin and oxytocin on the growth of MCF7 cells were assessed using protein accumulation and cell numbers. Vasopressin at 10-1000 pmol/liter was mitogenic for MCF7 cells, but higher doses (10 nmol/liter) were growth inhibitory. Oxytocin was also mitogenic for MCF7 cells but to a lesser extent than vasopressin. In conclusion, we suggest that vasopressin and possibly oxytocin may be important modulators of the growth of some human breast carcinomas.
采用放射免疫分析法测定了正常和癌性人乳腺组织中精氨酸加压素和催产素的含量。两种肽的含量均高于循环中的含量，但未发现正常组织和恶性组织之间存在差异。对人乳腺癌细胞（MCF7细胞）中[3H]催产素和[3H]加压素的结合进行了表征。两种激素与MCF7细胞的结合均具有特异性和饱和性，发现加压素受体为V1亚型。对数据进行Scatchard分析呈线性，表明每种激素都有一个单一的高亲和力、低容量结合位点（加压素：KD = 47.4 +/- 1.6 nmol/升，Bmax = 27,300 +/- 6,500位点/细胞；催产素：KD = 51.3 +/- 0.4 nmol/升，Bmax = 87,000 +/- 4,000位点/细胞）。使用蛋白质积累和细胞数量评估了加压素和催产素对MCF7细胞生长的影响。10 - 1000 pmol/升的加压素对MCF7细胞有促有丝分裂作用，但更高剂量（10 nmol/升）具有生长抑制作用。催产素对MCF7细胞也有促有丝分裂作用，但程度低于加压素。总之，我们认为加压素以及可能的催产素可能是某些人乳腺癌生长的重要调节因子。
