Garcin D, Massé T, Madjar J J, Jacquemont B
Ecole Normale Supérieure de Lyon, Institut National de la Santé et de la Recherche Médicale, France.
Eur J Biochem. 1990 Nov 26;194(1):279-86. doi: 10.1111/j.1432-1033.1990.tb19454.x.
Infection of human epidermoid carcinoma-2 (HEp-2) cells by Herpes simplex virus type 1 (HSV-1) leads to significant activation of inositol phospholipid turnover after 15 min. The effect of neomycin, an inhibitor of inositol phospholipid turnover, has been investigated for its effect on HSV-1 multiplication in HEp-2 cells. HSV-1 multiplication is inhibited by neomycin. This inhibition is not due to a block of virus adsorption or penetration. Neomycin inhibits the expression of virus immediate-early genes, as well as expression of early genes and viral DNA synthesis. In neomycin-treated cells, the usual virion-associated shut off of host protein synthesis does not occur. These results indicate that the inositol phospholipid pathway is involved in immediate-early gene expression and shut off of host protein synthesis in HEp-2 cells.
1型单纯疱疹病毒（HSV - 1）感染人表皮样癌2（HEp - 2）细胞15分钟后，会导致肌醇磷脂代谢显著激活。已研究了肌醇磷脂代谢抑制剂新霉素对HSV - 1在HEp - 2细胞中增殖的影响。新霉素可抑制HSV - 1的增殖。这种抑制并非由于病毒吸附或穿透受阻。新霉素抑制病毒立即早期基因的表达以及早期基因的表达和病毒DNA合成。在经新霉素处理的细胞中，通常与病毒粒子相关的宿主蛋白质合成关闭并未发生。这些结果表明，肌醇磷脂途径参与了HEp - 2细胞中立即早期基因的表达以及宿主蛋白质合成的关闭。
