Bøe Sigurd, Prasmickaite Lina, Engesæter Birgit, Hovig Eivind
Department of Tumor Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, N-0310, Norway.
Methods Mol Biol. 2011;764:107-21. doi: 10.1007/978-1-61779-188-8_7.
A major barrier within the field of non-viral gene therapy toward therapeutic strategies, e.g., tumor therapy, has been lack of appropriate specific delivery strategies to the intended target tissues or cells. In this chapter, we describe a protocol for light-directed delivery of nucleic acids through the use of photochemical internalization (PCI) technology. PCI is based on a photosensitizing compound that localizes to endocytic membranes. Upon illumination, the photosensitizing compound induces damage to the endocytic membranes, resulting in release of endocytosed material, i.e., nucleic acids into cytosol. The main benefit of the strategy described is the possibility for site-specific delivery of nucleic acids to a place of interest.
在非病毒基因治疗领域，针对诸如肿瘤治疗等治疗策略存在一个主要障碍，即缺乏针对目标组织或细胞的合适的特异性递送策略。在本章中，我们描述了一种通过使用光化学内化（PCI）技术进行光导向核酸递送的方案。PCI基于一种定位于内吞膜的光敏化合物。光照后，该光敏化合物会诱导内吞膜损伤，导致内吞物质（即核酸）释放到细胞质中。所述策略的主要优点是能够将核酸位点特异性递送至感兴趣的部位。
