Department of Tumor Biology, Institute for Cancer Research , The Norwegian Radium Hospital, Oslo, Norway .
Nucleic Acid Ther. 2013 Apr;23(2):131-9. doi: 10.1089/nat.2012.0403. Epub 2013 Feb 13.
A promising strategy for increased intracellular delivery of nucleic acids with the benefit for targeting is photochemical internalization (PCI). PCI relies on the use of a photosensitizing compound that photochemically destroys membranes in the endocytic pathway after illumination, resulting in cytosolic transfer of endosomal content. PCI technology combined with biodegradable polyamino acid carriers and nucleic acids delivers effective targeting and improved biosafety. In an in vitro model system, we have evaluated various poly-l-lysine (PLL), poly-l-histidine (PLH), and poly-l-arginine (PLA) formulations for light-directed small interference RNA (siRNA) gene silencing and messenger RNA (mRNA) delivery. We find that PLA formulations are suitable as siRNA and mRNA carriers in a strictly light-directed manner.
一种很有前途的增加核酸细胞内递送的策略是光化学内化（PCI）。PCI 依赖于使用光化学敏化化合物，该化合物在光照后在细胞内途径中光化学破坏膜，导致内体内容物的细胞质转移。PCI 技术与可生物降解的多氨基酸载体和核酸结合，可实现有效的靶向和提高生物安全性。在体外模型系统中，我们已经评估了各种聚赖氨酸（PLL）、聚组氨酸（PLH）和聚精氨酸（PLA）制剂在光导向小干扰 RNA（siRNA）基因沉默和信使 RNA（mRNA）递送上的作用。我们发现 PLA 制剂适合作为 siRNA 和 mRNA 的载体，并且严格依赖于光导向。
