Falasca Marco, Raimondi Claudio, Maffucci Tania
Barts and The London School of Medicine and Dentistry, Blizard Institute of Cell and Molecular Science, London, UK.
Methods Mol Biol. 2011;769:87-95. doi: 10.1007/978-1-61779-207-6_7.
The Boyden chamber, initially designed to study leukocyte chemotaxis, has become one of the most used tools to assess cell motility and invasion. The classical Boyden chamber consists of two compartments separated by a membrane representing a physical barrier that cells can overcome only by active migration. Since its initial introduction, a number of different Boyden chamber devices have been developed. The Boyden chamber can be adapted to study tumour cells' invasive properties by coating the membrane with different extracellular matrix proteins. The method described in this chapter is intended specifically for measuring the migration or invasion of human endothelial and cancer cells.
最初设计用于研究白细胞趋化性的博伊登小室，已成为评估细胞运动性和侵袭性最常用的工具之一。经典的博伊登小室由两个隔室组成，中间由一层膜隔开，该膜代表一种物理屏障，细胞只有通过主动迁移才能克服。自首次引入以来，已开发出许多不同的博伊登小室装置。通过用不同的细胞外基质蛋白包被膜，博伊登小室可用于研究肿瘤细胞的侵袭特性。本章所述方法专门用于测量人内皮细胞和癌细胞的迁移或侵袭。
