Sylvia A L, Rosenthal M
Brain Res. 1979 Apr 13;165(2):235-48. doi: 10.1016/0006-8993(79)90556-0.
Non-invasive optical techniques were used to monitor the effects of increasing cerebral energy demand on metabolic capabilities and vascular reactivity in young and aged brain. Low level of electrical stimulation of the cortex, in both young (4--7 months) and aged (24--28 months) rat brain, were accompanied by transient oxidations of NADH and cytochrome oxidase (a,a3) as measured by microfluorometry and reflection spectrophotometry respectively. Stimulation sufficient to produce spreading cortical depression was accompanied by an oxidation of both NADH and cytochrome a,a3 in young brain together with an increase in local blood volume. There was either no change or a slight disoxygenation of hemoglobin. In aged brain, however, spreading depression was associated with an oxidation of NADH and a reduction of cytochrome a,a3 together with an increase in local blood volume and an oxygenation of hemoglobin. The present results indicate that the relationship between microcirculation and the terminal oxidase step of the respiratory chain is altered in aged brain when energy demand is high.
采用非侵入性光学技术监测年轻和老年大脑中脑能量需求增加对代谢能力和血管反应性的影响。在年轻（4 - 7个月）和老年（24 - 28个月）大鼠大脑中，对皮层进行低水平电刺激时，分别通过显微荧光测定法和反射分光光度法测量发现，伴随着NADH和细胞色素氧化酶（a，a3）的短暂氧化。足以产生扩展性皮层抑制的刺激在年轻大脑中伴随着NADH和细胞色素a，a3的氧化以及局部血容量的增加，血红蛋白要么没有变化，要么有轻微的脱氧。然而，在老年大脑中，扩展性抑制与NADH的氧化、细胞色素a，a3的还原、局部血容量的增加以及血红蛋白的氧合有关。目前的结果表明，当能量需求较高时，老年大脑中微循环与呼吸链末端氧化酶步骤之间的关系发生了改变。
