Smyth J M, Sheahan B J, Atkins G J
Department of Microbiology, Moyne Institute, Trinity College, Dublin, Ireland.
J Gen Virol. 1990 Nov;71 ( Pt 11):2575-83. doi: 10.1099/0022-1317-71-11-2575.
The sites of multiplication in the mouse central nervous system (CNS) of the virulent L10 strain of Semliki Forest virus (SFV) and the L10-SFV-derived demyelinating M9 mutant were determined using both BALB/c and SJL mouse strains. In situ hybridization (ISH), using a cRNA probe to an SFV non-structural sequence, and immunogold-silver staining (IGSS), using polyclonal anti-SFV rabbit IgG, were the techniques utilized. For L10-SFV, viral RNA and antigen were detected in neurons and glial cells of both mouse strains. For BALB/c mice infected with M9-SFV, both neuronal and glial cell infection was less extensive than that obtained with L10. ISH or IGSS were generally not sensitive enough to detect viral RNA and antigen, respectively, in M9-SFV-infected SJL mice. M9-SFV multiplied to a similar titre in primary cultures of glial cells derived from either BALB/c or SJL mice. Following infection with M9-SFV, small plaques of demyelination in the CNS and occasional small aggregates of mononuclear leukocytes in the leptomeninges persisted for up to 12 months in SJL mice but not BALB/c mice. This was not associated with detectable persistence of infectious virus, viral antigen or viral RNA in the CNS.
利用BALB/c和SJL小鼠品系，确定了塞姆利基森林病毒（SFV）的强毒株L10和L10衍生的脱髓鞘M9突变体在小鼠中枢神经系统（CNS）中的增殖部位。使用针对SFV非结构序列的cRNA探针进行原位杂交（ISH），以及使用多克隆抗SFV兔IgG进行免疫金银染色（IGSS），是所采用的技术。对于L10 - SFV，在两种小鼠品系的神经元和胶质细胞中均检测到病毒RNA和抗原。对于感染M9 - SFV的BALB/c小鼠，神经元和胶质细胞感染的范围均不如L10感染时广泛。ISH或IGSS通常不够灵敏，无法分别在感染M9 - SFV的SJL小鼠中检测到病毒RNA和抗原。M9 - SFV在源自BALB/c或SJL小鼠的胶质细胞原代培养物中增殖至相似滴度。感染M9 - SFV后，SJL小鼠中枢神经系统中的小脱髓鞘斑块和软脑膜中偶尔出现的小单核白细胞聚集可持续长达12个月，而BALB/c小鼠则不会出现这种情况。这与中枢神经系统中可检测到的传染性病毒、病毒抗原或病毒RNA的持续存在无关。
