受体活性基因 1（Act-1）介导白细胞介素-17（IL-17）诱导类风湿关节炎成纤维样滑膜细胞表达晚期糖基化终产物受体（RAGE）。

The expression of the receptor for advanced glycation end-products (RAGE) in RA-FLS is induced by IL-17 via Act-1.

机构信息

The Rheumatism Research Center, Catholic Research Institute of Medical Science, The Catholic University of Korea, Seoul, 505 Banpo-dong, Seocho-gu, Seoul 137-040, South Korea.

出版信息

Arthritis Res Ther. 2011 Jul 12;13(4):R113. doi: 10.1186/ar3398.

INTRODUCTION

The receptor for advanced glycation end-products (RAGE) has been implicated in the pathogenesis of arthritis. We conducted this study to determine the effect of interleukin (IL)-17 on the expression and production of RAGE in fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). The role of nuclear factor-κB (NF-κB) activator 1 (Act1) in IL-17-induced RAGE expression in RA-FLS was also evaluated.

METHODS

RAGE expression in synovial tissues was assessed by immunohistochemical staining. RAGE mRNA production was determined by real-time polymerase chain reaction. Act-1 short hairpin RNA (shRNA) was produced and treated to evaluate the role of Act-1 on RAGE production.

RESULTS

RAGE, IL-17, and Act-1 expression increased in RA synovium compared to osteoarthritis synovium. RAGE expression and production increased by IL-17 and IL-1β (*P <0.05 vs. untreated cells) treatment but not by tumor necrosis factor (TNF)-α in RA-FLS. The combined stimuli of both IL-17 and IL-1β significantly increased RAGE production compared to a single stimulus with IL-17 or IL-1β alone (P <0.05 vs. 10 ng/ml IL-17). Act-1 shRNA added to the RA-FLS culture supernatant completely suppressed the enhanced production of RAGE induced by IL-17.

CONCLUSIONS

RAGE was overexpressed in RA synovial tissues, and RAGE production was stimulated by IL-17 and IL-1β. Act-1 contributed to the stimulatory effect of IL-17 on RAGE production, suggesting a possible inhibitory target for RA treatment.

简介

晚期糖基化终产物受体（RAGE）已被认为与关节炎的发病机制有关。我们进行了这项研究，以确定白细胞介素（IL）-17 对类风湿关节炎（RA）成纤维样滑膜细胞（FLS）中 RAGE 的表达和产生的影响。还评估了核因子-κB（NF-κB）激活物 1（Act1）在 RA-FLS 中 IL-17 诱导的 RAGE 表达中的作用。

方法

通过免疫组织化学染色评估滑膜组织中 RAGE 的表达。通过实时聚合酶链反应确定 RAGE mRNA 的产生。产生 Act1 短发夹 RNA（shRNA）并进行处理，以评估 Act1 在 RAGE 产生中的作用。

结果

与骨关节炎滑膜相比，RA 滑膜中 RAGE、IL-17 和 Act1 的表达增加。IL-17 和 IL-1β（*P <0.05 与未处理细胞相比）处理可增加 RA-FLS 中的 RAGE 表达和产生，但 TNF-α 则不能。与单独使用 IL-17 或 IL-1β 相比，IL-17 和 IL-1β 的联合刺激可显著增加 RAGE 的产生（P <0.05 与 10 ng/ml IL-17 相比）。将 Act1 shRNA 添加到 RA-FLS 培养上清液中可完全抑制由 IL-17 诱导的 RAGE 产生的增强。