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一种用于分离霍乱弧菌O1的新型选择性鉴别琼脂培养基:多粘菌素-甘露糖-亚碲酸盐琼脂(PMT琼脂)。

A new selective, differential agar medium for isolation of Vibrio cholerae O1: PMT (polymyxin-mannose-tellurite) agar.

作者信息

Shimada T, Sakazaki R, Fujimura S, Niwano K, Mishina M, Takizawa K

机构信息

Department of Bacteriology, National Institute of Health, Tokyo.

出版信息

Jpn J Med Sci Biol. 1990 Apr;43(2):37-41. doi: 10.7883/yoken1952.43.37.

Abstract

A new selective and differential agar medium, polymyxin-mannose-tellurite (PMT) agar was devised to differentiate easily colonies of Vibrio cholerae O1 from those of V. cholerae non-O1. The differentiation between colonies of the two vibrios is based on mannose-fermentation. Colonies of V. cholerae O1 on the agar are agglutinated with O1 antiserum of V. cholerae much more easily than those on thiosulfate-citrate-bile salts-sucrose (TCBS) agar.

摘要

一种新的选择性鉴别琼脂培养基——多粘菌素-甘露糖-亚碲酸盐(PMT)琼脂被设计出来,用于轻松区分霍乱弧菌O1群菌落与非O1群霍乱弧菌菌落。两种弧菌菌落之间的区分基于甘露糖发酵。琼脂上霍乱弧菌O1群的菌落比在硫代硫酸盐-柠檬酸盐-胆盐-蔗糖(TCBS)琼脂上的菌落更容易被霍乱弧菌O1抗血清凝集。

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