氯化钠增强硫代硫酸盐柠檬酸盐胆盐蔗糖琼脂对霍乱弧菌埃尔托生物型的分离选择性。
Enhancement by sodium chloride of the selectivity of thiosulfate citrate bile salts sucrose agar for isolating Vibrio cholerae biotype El Tor.
作者信息
Morris G K, DeWitt W E, Gangarosa E J, McCormack W M
出版信息
J Clin Microbiol. 1976 Aug;4(2):133-6. doi: 10.1128/jcm.4.2.133-136.1976.
Abstract
In this study we utilized the salt-tolerant characteristics of vibrios to develop a more selective medium by addition of NaCl to thiosulfate citrate bile salts sucrose (TCBS) agar. The effect of adding salt to TCBS agar varied greatly among brands of TCBS agar and between lots of the same brand. The addition of salt at concentrations as high as 1.5% (2.5% total NaCl) caused the inhibition of growth of three species of commonly encountered normal bowel flora and one strain of classical Vibrio cholerae but did not compromise the use of TCBS agar for isolation of V. cholerae biotype El Tor.
摘要
在本研究中，我们利用弧菌的耐盐特性，通过向硫代硫酸盐柠檬酸盐胆盐蔗糖（TCBS）琼脂中添加氯化钠来开发一种更具选择性的培养基。向TCBS琼脂中添加盐的效果在不同品牌的TCBS琼脂之间以及同一品牌的不同批次之间差异很大。添加浓度高达1.5%（总氯化钠含量为2.5%）的盐会抑制三种常见的正常肠道菌群和一株经典霍乱弧菌的生长，但不会影响使用TCBS琼脂分离霍乱弧菌埃尔托生物型。
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