Musculoskeletal Research Group, Institute of Anatomy, Ludwig-Maximilian-University Munich, Munich, Germany.
Histol Histopathol. 2011 Sep;26(9):1173-85. doi: 10.14670/HH-26.1173.
Tendons have a limited capacity for self-repair due to the low density and mitotic activity of tenocytes. Pro-inflammatory cytokines such as interleukin-1β (IL-1β) have been identified as the main initiators of tendinopathies, stimulating inflammation, apoptosis and extracellular matrix (ECM) degradation. The aim of this study was to evaluate the potential of Tendoactive®, a newly developed proprietary nutraceutical formulation that includes mucopolysaccharides, collagen and vitamin C, in an in vitro model of tendon inflammation. The effects of Tendoactive® were studied in primary cultures of human tenocytes treated with IL-1β for up to 72 h. Expression of collagen type I, integrin β1, cyclo-oxygenase-2 (COX-2), caspase-3 and matrix metalloproteinase-1 (MMP-1) was monitored by western blotting. The effects of Tendoactive® on the expression, phosphorylation and nuclear translocation of protein components of the NF-κB system were studied by western blotting and immunofluorescence respectively. Treatment of tenocytes with Tendoactive® suppressed IL-1β-induced NF-κB activation and p65 nuclear translocation. These events correlated with down-regulation of NF-κB targets including COX-2, MMP-1 and activated caspase-3. Tendoactive® also reversed the IL-1β-induced down-regulation of collagen type I and β1-integrin receptor expression. These results indicate that Tendoactive® has nutraceutical potential as an anti-inflammatory agent for treating tendinopathy through suppression of NF-κB mediated IL-1β catabolic signalling pathways in tenocytes.
由于肌腱细胞的密度低和有丝分裂活性低，肌腱的自我修复能力有限。促炎细胞因子，如白细胞介素-1β（IL-1β），已被确定为肌腱病的主要启动子，刺激炎症、细胞凋亡和细胞外基质（ECM）降解。本研究旨在评估一种新开发的专有营养配方 Tendoactive®在肌腱炎症的体外模型中的潜力。该配方包括粘多糖、胶原蛋白和维生素 C。研究了 Tendoactive®在经 IL-1β处理的人肌腱细胞原代培养物中长达 72 小时的作用。通过 Western blot 监测胶原蛋白 I 型、整合素β1、环氧化酶-2（COX-2）、半胱天冬酶-3 和基质金属蛋白酶-1（MMP-1）的表达。通过 Western blot 和免疫荧光分别研究了 Tendoactive®对 NF-κB 系统蛋白成分表达、磷酸化和核易位的影响。用 Tendoactive®处理肌腱细胞可抑制 IL-1β诱导的 NF-κB 激活和 p65 核易位。这些事件与 NF-κB 靶标（包括 COX-2、MMP-1 和活化的半胱天冬酶-3）的下调相关。Tendoactive®还逆转了 IL-1β诱导的胶原蛋白 I 和β1-整合素受体表达下调。这些结果表明，Tendoactive®具有营养潜力，可作为一种抗炎剂，通过抑制 NF-κB 介导的肌腱细胞中 IL-1β分解代谢信号通路来治疗肌腱病。
