Xue Cai-fu, Guo Jian-ming, Qian Da-wei, Duan Jin-ao, Shu Yan
Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Yao Xue Xue Bao. 2011 Apr;46(4):454-9.
To explore the mechanism of the absorption of flavonoids from Abelmoschus manihot flowers, in situ intestinal recirculation was performed to study the effect of the absorption at different concentrations and different intestinal regions. To evaluate the conditions of the absorption of six flavonoids from Abelmoschus manihot flowers, the concentrations of Abelmoschus manihot in the perfusion solution were determined by HPLC at predesigned time. And we have investigated the inhibitory effect of six flavonoids from Abelmoschus manihot flowers on P-glycoprotein (P-gp) drug efflux pump. The results demonstrated that the absorption rates of flavonoids from Abelmoschus manihot flowers are not significantly different (P > 0.05) at various drug concentrations, the absorption of flavonoids from Abelmoschus manihot flowers is a first-order process with the passive diffusion mechanism. The absorption rates of each of flavonoids are significantly different. The absorption rate of flavonoid glycoside was lower than that of aglycone; the flavonoids from Abelmoschus manihot flowers could be absorbed in all of the intestinal segments. The best parts of intestine to absorb hyperoside and myricetin are jejunum and duodenum, separately. Verapamil could enhance the absorption of isoquercitrin, hyperoside, myricetin and quercetin-3'-O-glucoside by inhibiting P-glycoprotein (P-gp) drug efflux pump.
为探究黄蜀葵花中黄酮类成分的吸收机制，采用在体肠循环法研究不同浓度及不同肠段对其吸收的影响。通过高效液相色谱法（HPLC）在预设时间测定灌流液中黄蜀葵花的浓度，以评价黄蜀葵花中6种黄酮类成分的吸收情况。并考察了黄蜀葵花中6种黄酮类成分对P-糖蛋白（P-gp）药物外排泵的抑制作用。结果表明，不同药物浓度下黄蜀葵花中黄酮类成分的吸收速率差异无统计学意义（P>0.05），黄蜀葵花中黄酮类成分的吸收为一级过程，吸收机制为被动扩散。各黄酮类成分的吸收速率存在显著差异，黄酮苷的吸收速率低于苷元；黄蜀葵花中黄酮类成分在各肠段均可吸收，其中金丝桃苷和杨梅素的最佳吸收肠段分别为空肠和十二指肠。维拉帕米可通过抑制P-糖蛋白（P-gp）药物外排泵增强异槲皮苷、金丝桃苷、杨梅素和槲皮素-3'-O-葡萄糖苷的吸收。
