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[黄蜀葵不同组织中多种化学成分的分析及利用价值探讨]

[Analysis and utilization value discussion of multiple chemical composition in different tissues of Abelmoschus manihot].

作者信息

Liu Jie, Guo Sheng, Duan Jin-Ao, Yan Hui, Qian Da-Wei, Tang Hai-Tao, Tang Ren-Mao

机构信息

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, State Administration of Traditional Chinese Medicine Key Laboratory of Chinese Medicinal Resources Recycling Utilization, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.

SZYY Group Pharmaceutical Ltd., Taizhou 225500, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2016 Oct;41(20):3782-3791. doi: 10.4268/cjcmm20162013.

Abstract

This research is to analyze the resourceful chemical composition in different tissues (root, stem, leaf and flower) of Abelmoschus manihot and evaluate their utilizing value. The flavonoids, soluble polysaccharides, cellulose, nucleosides and amino acids in the different tissues of A. manihot were determined by HPLC coupled with UV-Vis spectrophotpmetry, and UPLC-TQ/MS. The flowers are rich in the resourceful chemical compositions of flavonoids which mainly consist of hyperoside, isoquercitrin, cotton-8-O-glucuronide, myricetin, quercetin-3'-O-glucoside, rutin and quercetin. The total content of these flavonoids is 25.450 mg•g-1 in the flowers, while they are trace in the other tissues.Different tissues of A. manihot are rich in soluble polysaccharides and celluloses and the stems have the highest content(19.76%) of soluble polysaccharides, while the roots have the highest content (29.88%) of cellulose. Total of 21 amino acids and 9 nucleosides were detected in this plant, and the flowers have the highest content of amino acids(4.737 mg•g⁻¹), while the leaves have the highest content of nucleosides (1.474 mg•g⁻¹). A. manihot is rich in the resourceful chemical compositions, and its constituents and contents are various in different tissues of this plant.The results provided a scientific basis for the utilization and industrial development of A. manihot plants.

摘要

本研究旨在分析黄蜀葵不同组织(根、茎、叶和花)中丰富的化学成分,并评估其利用价值。采用高效液相色谱-紫外可见分光光度法以及超高效液相色谱-串联四极杆质谱法测定了黄蜀葵不同组织中的黄酮类化合物、可溶性多糖、纤维素、核苷和氨基酸。花中富含黄酮类化合物这种丰富的化学成分,主要包括金丝桃苷、异槲皮苷、棉8-O-葡萄糖醛酸苷、杨梅素、槲皮素-3'-O-葡萄糖苷、芦丁和槲皮素。这些黄酮类化合物在花中的总含量为25.450 mg•g-1,而在其他组织中含量甚微。黄蜀葵的不同组织富含可溶性多糖和纤维素,茎中可溶性多糖含量最高(19.76%),而根中纤维素含量最高(29.88%)。该植物共检测到21种氨基酸和9种核苷,花中氨基酸含量最高(4.737 mg•g⁻¹),而叶中核苷含量最高(1.474 mg•g⁻¹)。黄蜀葵富含丰富的化学成分,其成分和含量在该植物的不同组织中各不相同。研究结果为黄蜀葵植物的利用和产业发展提供了科学依据。

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