Pan Xinxin, Du Leyue, Tao Jinhua, Jiang Shu, Qian Dawei, Duan Jinao
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, PR China.
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Aug 1;1059:21-26. doi: 10.1016/j.jchromb.2017.05.020. Epub 2017 May 23.
Abelmoschus manihot (Linn.) Medicus has been clinically used to treat chronic kidney disease, oral ulcers, burns, and dysmenorrhea in China for many centuries. The major pharmacologically-active components of A. manihot are flavonoids. In this study, a rapid and highly sensitive UPLC-MS/MS analysis method was established and successfully applied to the simultaneous determination of five major flavonoids (rutin, hyperoside, isoquercitrin, quercetin, and myricetin) in different parts of A. manihot harvested at ten growth periods. Under the optimized chromatographic conditions, good separation for five target components was obtained on an Acquity UPLC BEH C18 column within 18min. The total contents of the five investigated flavonoids in A. manihot roots, stems, leaves and flowers ranged from 2.86 to 123.7μg/g, 46.39 to 141.0μg/g, 929.4 to 3096μg/g, and 10,150 to 19,390μg/g, respectively, indicating that the total flavonoids in the four parts could be mainly arranged in a decreasing order as flower>leaf>stem>root. The peak of total flavonoids in flowers and leaves appeared at G8 and G9, respectively. These results will be helpful for the determination of the suitable harvest time of A. manihot and the improvement of the utility value of the disused parts.
黄蜀葵在中国临床上用于治疗慢性肾病、口腔溃疡、烧伤和痛经已有数百年历史。黄蜀葵的主要药理活性成分是黄酮类化合物。本研究建立了一种快速、高灵敏度的超高效液相色谱-串联质谱（UPLC-MS/MS）分析方法，并成功应用于同时测定十个生长时期收获的黄蜀葵不同部位中的五种主要黄酮类化合物（芦丁、金丝桃苷、异槲皮苷、槲皮素和杨梅素）。在优化的色谱条件下，在Acquity UPLC BEH C18柱上18分钟内可实现五种目标成分的良好分离。黄蜀葵根、茎、叶和花中五种被测黄酮类化合物的总含量分别为2.86至123.7μg/g、46.39至141.0μg/g、929.4至3096μg/g和10150至19390μg/g，表明这四个部位中的总黄酮含量主要呈花>叶>茎>根的递减顺序排列。花和叶中总黄酮的峰值分别出现在G8和G9期。这些结果将有助于确定黄蜀葵的适宜采收期，并提高废弃部位的利用价值。
