Allen E M, Weir J P, Martin S, Mercadal C, Rouse B T
Department of Microbiology, University of Tennessee, Knoxville.
Viral Immunol. 1990 Fall;3(3):207-15. doi: 10.1089/vim.1990.3.207.
Vaccinia virus recombinants co-expressing the genes for IL-2 and HSV-1 gC or gD were used to study the potential adjuvant effects of IL-2 on immunity to HSV-1. In addition, constructs were produced which, while expressing IL-2, encoded for reduced levels of HSV-1 gC. Studies with mice revealed that gC and gD could stimulate HSV-1 immunity as measured by neutralizing antibody (NA), lymphoproliferation and viral clearance. IL-2 enhancement of NA and lymphoproliferation was observed only in those groups of mice immunized with the sub-optimal gC construct which co-expressed IL-2. In no instance did the presence of IL-2 augment the ability of mice to clear an epithelial challenge of HSV-1.
共表达白细胞介素-2（IL-2）基因和单纯疱疹病毒1型（HSV-1）糖蛋白C（gC）或糖蛋白D（gD）的痘苗病毒重组体被用于研究IL-2对HSV-1免疫的潜在佐剂效应。此外，构建了在表达IL-2的同时编码较低水平HSV-1 gC的载体。对小鼠的研究表明，通过中和抗体（NA）、淋巴细胞增殖和病毒清除来衡量，gC和gD可刺激HSV-1免疫。仅在用共表达IL-2的次优gC构建体免疫的小鼠组中观察到IL-2增强了NA和淋巴细胞增殖。在任何情况下，IL-2的存在都没有增强小鼠清除HSV-1上皮攻击的能力。
