Forrester A J, Sullivan V, Simmons A, Blacklaws B A, Smith G L, Nash A A, Minson A C
Department of Pathology, University of Cambridge, U.K.
J Gen Virol. 1991 Feb;72 ( Pt 2):369-75. doi: 10.1099/0022-1317-72-2-369.
Passive administration of neutralizing monoclonal antibody (MAb) to glycoprotein H (gH) of herpes simplex virus type 1 (HSV-1) was found to protect mice from an HSV-1 strain SC16 challenge infection. To investigate further the protective potential of gH, recombinant vaccinia viruses were constructed which expressed the HSV-1 gH open reading frame under the control of the vaccinia virus 7.5K early/late promoter or the 4b late promoter. Immunization with recombinant viruses, however, did not induce the production of neutralizing antisera and the mice were not protected from zosteriform spread or the establishment of latent infection following viral challenge. The gH produced by the recombinant vaccinia viruses differed in electrophoretic mobility and antigenicity from authentic HSV-1 gH. Only one of three neutralizing MAbs specific for conformational epitopes on gH was able to immunoprecipitate gH synthesized in recombinant vaccinia virus-infected cells. In addition cell surface expression of gH was not detected in cells infected with the recombinant vaccinia viruses.
发现向1型单纯疱疹病毒（HSV-1）的糖蛋白H（gH）被动给予中和单克隆抗体（MAb）可保护小鼠免受HSV-1毒株SC16的攻击感染。为了进一步研究gH的保护潜力，构建了重组痘苗病毒，其在痘苗病毒7.5K早期/晚期启动子或4b晚期启动子的控制下表达HSV-1 gH开放阅读框。然而，用重组病毒免疫并未诱导产生中和抗血清，并且在病毒攻击后，小鼠未免受带状疱疹样传播或潜伏感染的建立。重组痘苗病毒产生的gH在电泳迁移率和抗原性方面与天然HSV-1 gH不同。针对gH上构象表位的三种中和MAb中只有一种能够免疫沉淀在重组痘苗病毒感染细胞中合成的gH。此外，在感染重组痘苗病毒的细胞中未检测到gH的细胞表面表达。
